engleski

Mixed Adjuvant Formulation Based on Peptide Hydrogel and Desmuramyl Peptides: Preparation and Influence on Specific Immune Reaction

Adjuvants are essential for enhancing vaccine potency by improvement of the humoral and cell­ mediated immune response to vaccine antigens. Adjuvants of new generation are adjuvant systems composed of various combinations of classical adjuvants designed to adjust the adaptive immune responses against pathogens. The challenge for this strategy is to define an effective and safe formulation in which individual components can synergize with one another to elicit a more robust immune response. Self­assembling peptides have a great potential for applications in bionanotechnology and it has been shown that certain peptides possess immunostimulatory activity (1). Muramyl dipeptide (MDP) is a synthetic immunoactive peptide consisting of N­ acetyl muramic acid attached to a dipeptide L­Ala­D­ isoGln. It was first identified in bacterial cell wall peptidoglycan as the smallest fragment possessing adjuvant activity. Desmuramylpeptides have been extensively studied in an attempt to increase adjuvant activity and boost the immune response effectively for clinical use in the treatment of cancer and other diseases. We report here the preparation and characterization of adjuvant system based on supramolecular hydrogel of a self­assembling tripeptide Ac­L­Phe­L­Phe­L­Ala­NH2 with built­in desmuramylpeptide immunopotentiators (2, 3). Recently, a series of novel acyl tripeptides mimicking MDP were identified as potent nanomolar NOD2 agonists. The most potent derivatives were incorporated into hydrogel and tested in the mouse model of adjuvancy. The obtained results show that adjuvant system based on a hydrogel of a self­assembling tripeptide Ac­L­ Phe­L­Phe­L­Ala­NH2 with built­in desmuramylpeptides elicits stronger specific immune response in comparison with antigen and adjuvant alone.

Adjuvant ; peptide hydrogel ; Desmuramyl peptide ; immune reaction ;

nije evidentirano