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Pregled bibliografske jedinice broj: 1194474

Method for assessment of intracellular level of cadmium and thallium


Krivohlavek, A; Čoklo, M; Kuharic, Z; Šabaric, J; Sikic, S; Marjanovic Cermak, AM; Pavicic, I; Domijan, A-M
Method for assessment of intracellular level of cadmium and thallium // Abstracts of the 55th Congress of the European Societies of Toxicology (EUROTOX2019) TOXICOLOGY-SCIENCE PROVIDING SOLUTIONS / Dekant, Wolfgang (ur.).
Helsinki, Finska: Elsevier, 2019. str. S176-S177 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1194474 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Method for assessment of intracellular level of cadmium and thallium

Autori
Krivohlavek, A ; Čoklo, M ; Kuharic, Z ; Šabaric, J ; Sikic, S ; Marjanovic Cermak, AM ; Pavicic, I ; Domijan, A-M

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 55th Congress of the European Societies of Toxicology (EUROTOX2019) TOXICOLOGY-SCIENCE PROVIDING SOLUTIONS / Dekant, Wolfgang - : Elsevier, 2019, S176-S177

Skup
55th Congress of the European Societies of Toxicology (EUROTOX2019) TOXICOLOGY-SCIENCE PROVIDING SOLUTIONS

Mjesto i datum
Helsinki, Finska, 08-11.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HepG2 stanice ; ICP-MS ; teški metali
(HepG2 cells ; ICP-MS ; heavy metals)

Sažetak
Cadmium (Cd) and thallium (Tl) are highly toxic metals. Their deleterious effect on human health is well established, and their mechanisms of toxicity are still explored on in vitro model. To accurately link specific change in the cell with the exposure to metals, the intracellular level of metal should be determined. Therefore the aim of this study was to develop method that will enable accurate determination of intracellular level of Cd and Tl of cells treated with these two metals. Cells (HepG2 ; at planting density of 2.5x105 cells/mL) were treated with Cd or Tl (1 and 10 mg/L) for 24 h. Afterwards, cells were washed in PBS buffer and resuspended in 0.5 mL of PBS buffer. Cells were digested with equal volume of 2% HNO3. For monitoring intracellular level of Cd and Tl, ICP-MS (ELAN DRC-e, Perkin Elmer, Singapore 2008) was applied. For validation experiments Cd and Tl standards as well as reference materials CRM DORMA 4 Fish protein (NRC Canada) for Cd and NCS ZC 73028 Rice (China National Analysis Center) for Tl were used. In concentration range tested (0.2–200 μ g/L) developed method was linear and correlation coefficient (R2) for Cd was 0.9999 with intercept 0.0732 while for Tl was 1.00009 with intercept - 0.1497. Limit of detection (LOD), repeatability and trueness for Cd were 0.020 μg/L, 2.9%, 96.6% and for Tl 0.097 μg/L, 3.7% and 102%, respectively. Developed method was applied to cells treated with Cd or Tl. The intracellular level of Cd and Tl was concentrationdependent. At higher concentration higher intracellular level of Cd and Tl were detected. However, higher uptake of Cd in comparison to Tl was observed (at concentration level 1 mg/L ; 10% for Cd in comparison to 1% for Tl) that can explain higher toxicity of Cd to cells. In conclusion, validation parameters indicate that developed method is reliable and accurate for intracellular assessment of Cd and Tl and enabled following low levels of Cd and Tl within the cell. Furthermore, from obtained results it can be concluded that cells more readily accumulate Cd than Tl.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Nastavni zavod za javno zdravstvo "Dr. Andrija Štampar"


Citiraj ovu publikaciju:

Krivohlavek, A; Čoklo, M; Kuharic, Z; Šabaric, J; Sikic, S; Marjanovic Cermak, AM; Pavicic, I; Domijan, A-M
Method for assessment of intracellular level of cadmium and thallium // Abstracts of the 55th Congress of the European Societies of Toxicology (EUROTOX2019) TOXICOLOGY-SCIENCE PROVIDING SOLUTIONS / Dekant, Wolfgang (ur.).
Helsinki, Finska: Elsevier, 2019. str. S176-S177 (poster, međunarodna recenzija, sažetak, znanstveni)
Krivohlavek, A., Čoklo, M., Kuharic, Z., Šabaric, J., Sikic, S., Marjanovic Cermak, A., Pavicic, I. & Domijan, A. (2019) Method for assessment of intracellular level of cadmium and thallium. U: Dekant, W. (ur.)Abstracts of the 55th Congress of the European Societies of Toxicology (EUROTOX2019) TOXICOLOGY-SCIENCE PROVIDING SOLUTIONS.
@article{article, author = {Krivohlavek, A and \v{C}oklo, M and Kuharic, Z and \v{S}abaric, J and Sikic, S and Marjanovic Cermak, AM and Pavicic, I and Domijan, A-M}, editor = {Dekant, W.}, year = {2019}, pages = {S176-S177}, keywords = {HepG2 stanice, ICP-MS, te\v{s}ki metali}, title = {Method for assessment of intracellular level of cadmium and thallium}, keyword = {HepG2 stanice, ICP-MS, te\v{s}ki metali}, publisher = {Elsevier}, publisherplace = {Helsinki, Finska} }
@article{article, author = {Krivohlavek, A and \v{C}oklo, M and Kuharic, Z and \v{S}abaric, J and Sikic, S and Marjanovic Cermak, AM and Pavicic, I and Domijan, A-M}, editor = {Dekant, W.}, year = {2019}, pages = {S176-S177}, keywords = {HepG2 cells, ICP-MS, heavy metals}, title = {Method for assessment of intracellular level of cadmium and thallium}, keyword = {HepG2 cells, ICP-MS, heavy metals}, publisher = {Elsevier}, publisherplace = {Helsinki, Finska} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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