In vitro effect of gangliosides on lipid peroxidation in human spermatozoa (CROSBI ID 739318)
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Podaci o odgovornosti
Gavella, Mirjana ; Rakoš, Romina ; Lipovac, Vaskresenija
engleski
In vitro effect of gangliosides on lipid peroxidation in human spermatozoa
Introduction: Lipid peroxidation-induced changes in physical properties of sperm membrane may have deleterious consequences on spermatozoa functioning. Since gangliosides, a sialic acid containing glycosphingolipids, were shown to have inhibitory effect on lipid peroxidation (LPO) in several cell types, we examined the effect of gangliosides on lipid peroxidation in the spermatozoa. Methods: The in vitro effect of gangliosides on LPO induced by 0.2mM ferrous sulphate/1mM ascorbate system in spermatozoa from 42 infertile men was investigated. The study was approved by the ethical committee at the Vuk Vrhovac University Clinic. The sperm concentration (mean+-SD) was 83.3+-65.1x10^6/ml ; motility 53.8+-18.3% ; progressive motility 35.6+-15.2% ; vitality 62.0+-16.0% ; hypo-osmotic swelling test 51.5+-12.2% and leukocytes <1x10^6/ml. Experimentally induced LPO was performed for 60 min at 37^oC with and without gangliosides (disialoganglioside GD1a and trisialoganglioside GT1b, Sigma, USA). Accumulation of malondialdehide (MDA) was estimated spectrophotometrically using thiobarbituric acid-reactive substances (TBARS)assay. Results and Discussion: Initial experiments have shown that the reduction of MDA generation was obtained only in the presence of more polar GT1b (40.6+-9.9 vs.27.3+-6.6 nmol TBARS/10^8 sperms in the presence of GT1b ; n=8)whereas an equal cocentration of GD1a(100uM) did not reveal any influence on LPO (39.9+-11.6 vs. 39.1+-10.5 nmol/10^8 sperms). GT1b affected LPO in a concentration-dependent manner (23, 33 and 55% in the presence of 25, 50 and 100 uM GT1b, respectively). GT1b (100uM) significantly inhibited LPO in the spermatozoa of 42 infertile men (29.7+-11.3 nmol TBARS/10^8 sperms without gangliosides vs. 23.2+-6.9 nmol/10^8 sperms in the presence of GT1b ; p<0.0001). This study shows that the application of extracellular gangliosides provides some protection to spermatozoa against LPO, probably being related to stabilizing effect on sperm membrane.
sperm membrane ; human spermatozoa ; lipid peroxidation ; gangliosides
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Podaci o prilogu
84--.
2003.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Reproduction. Abstract series
1476-3990
Podaci o skupu
Nepoznat skup
ostalo
29.02.1904-29.02.2096