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Pregled bibliografske jedinice broj: 1194160

Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment


Kvesić, Marija; Šamanić, Ivica; Novak, Anita; Fredotović, Željana; Dželalija, Mia; Kamenjarin, Juraj; Goić Barišić, Ivana; Tonkić, Marija; Maravić, Ana
Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment // Frontiers in Microbiology, 13 (2022), 858821, 14 doi:10.3389/fmicb.2022.858821 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1194160 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment

Autori
Kvesić, Marija ; Šamanić, Ivica ; Novak, Anita ; Fredotović, Željana ; Dželalija, Mia ; Kamenjarin, Juraj ; Goić Barišić, Ivana ; Tonkić, Marija ; Maravić, Ana

Izvornik
Frontiers in Microbiology (1664-302X) 13 (2022); 858821, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antibiotic resistance ; KPC ; OXA-48 ; Enterobacteriaceae

Sažetak
The rapid and ongoing spread of carbapenemase- producing Enterobacteriaceae has led to a global health threat. However, a limited number of studies have addressed this problem in the marine environment. We investigated their emergence in the coastal waters of the central Adriatic Sea (Croatia), which are recipients of submarine effluents from two wastewater treatment plants. Fifteen KPC-producing Enterobacteriaceae (nine Escherichia coli, four Klebsiella pneumoniae and two Citrobacter freundii) were recovered, and susceptibility testing to 14 antimicrobials from 10 classes showed that four isolates were extensively drug resistant (XDR) and two were resistant to colistin. After ERIC and BOX-PCR typing, eight isolates were selected for whole genome sequencing. The E. coli isolates belonged to serotype O21:H27 and sequence type (ST) 2795, while K. pneumoniae isolates were assigned to STs 37 and 534. Large-scale genome analysis revealed an arsenal of 137 genes conferring resistance to 19 antimicrobial drug classes, 35 genes associated with virulence, and 20 plasmid replicons. The isolates simultaneously carried 43–90 genes encoding for antibiotic resistance, while four isolates co-harbored carbapenemase genes blaKPC-2 and blaOXA-48. The blaOXA-48 was associated with IncL-type plasmids in E. coli and K. pneumoniae. Importantly, the blaKPC-2 in four E. coli isolates was located on ~40 kb IncP6 broad-host-range plasmids which recently emerged as blaKPC-2 vesicles, providing first report of these blaKPC- 2-bearing resistance plasmids circulating in E. coli in Europe. This study also represents the first evidence of XDR and potentially virulent strains of KPC-producing E. coli in coastal waters and the co-occurrence of blaKPC-2 and blaOXA-48 carbapenemase genes in this species. The leakage of these strains through submarine effluents into coastal waters is of concern, indicating a reservoir of this infectious threat in the marine environment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-UIP-2019-04-9778 - Sezonska i prostorna raspodjela gena rezistencije na antibiotike u morskim mikrobnim zajednicama duž trofičkog gradijenta u srednjem Jadranu (ARGAS) (Maravić, Ana, HRZZ - 2019-04) ( POIROT)
EK-KF-KK.01.1.1.01.0003 - STIM-REI (STIM-REI) (Bonačić-Koutecky, Vlasta; Smital, Tvrtko; Orlić, Sandi, EK - KK.01.1.1.01) ( POIROT)
EK-EFRR-KK.01.1.1.04.0064 - Razvoj tehnologije za procjenu autopurifikacijskih sposobnosti priobalnih voda (CAAT) (Andričević, Roko, EK - KK.01.1.1.04. Ulaganje u znanost i inovacije-Prvi poziv) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Split,
Sveučilište u Splitu

Poveznice na cjeloviti tekst rada:

doi www.frontiersin.org

Citiraj ovu publikaciju:

Kvesić, Marija; Šamanić, Ivica; Novak, Anita; Fredotović, Željana; Dželalija, Mia; Kamenjarin, Juraj; Goić Barišić, Ivana; Tonkić, Marija; Maravić, Ana
Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment // Frontiers in Microbiology, 13 (2022), 858821, 14 doi:10.3389/fmicb.2022.858821 (međunarodna recenzija, članak, znanstveni)
Kvesić, M., Šamanić, I., Novak, A., Fredotović, Ž., Dželalija, M., Kamenjarin, J., Goić Barišić, I., Tonkić, M. & Maravić, A. (2022) Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment. Frontiers in Microbiology, 13, 858821, 14 doi:10.3389/fmicb.2022.858821.
@article{article, author = {Kvesi\'{c}, Marija and \v{S}amani\'{c}, Ivica and Novak, Anita and Fredotovi\'{c}, \v{Z}eljana and D\v{z}elalija, Mia and Kamenjarin, Juraj and Goi\'{c} Bari\v{s}i\'{c}, Ivana and Tonki\'{c}, Marija and Maravi\'{c}, Ana}, year = {2022}, pages = {14}, DOI = {10.3389/fmicb.2022.858821}, chapter = {858821}, keywords = {Antibiotic resistance, KPC, OXA-48, Enterobacteriaceae}, journal = {Frontiers in Microbiology}, doi = {10.3389/fmicb.2022.858821}, volume = {13}, issn = {1664-302X}, title = {Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment}, keyword = {Antibiotic resistance, KPC, OXA-48, Enterobacteriaceae}, chapternumber = {858821} }
@article{article, author = {Kvesi\'{c}, Marija and \v{S}amani\'{c}, Ivica and Novak, Anita and Fredotovi\'{c}, \v{Z}eljana and D\v{z}elalija, Mia and Kamenjarin, Juraj and Goi\'{c} Bari\v{s}i\'{c}, Ivana and Tonki\'{c}, Marija and Maravi\'{c}, Ana}, year = {2022}, pages = {14}, DOI = {10.3389/fmicb.2022.858821}, chapter = {858821}, keywords = {Antibiotic resistance, KPC, OXA-48, Enterobacteriaceae}, journal = {Frontiers in Microbiology}, doi = {10.3389/fmicb.2022.858821}, volume = {13}, issn = {1664-302X}, title = {Submarine Outfalls of Treated Wastewater Effluents are Sources of Extensively- and Multidrug- Resistant KPC- and OXA-48-Producing Enterobacteriaceae in Coastal Marine Environment}, keyword = {Antibiotic resistance, KPC, OXA-48, Enterobacteriaceae}, chapternumber = {858821} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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