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Pregled bibliografske jedinice broj: 1193493

Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus


Weitner, Tin; Friganović, Tomislav; Šakić, Davor
Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus // Analytical chemistry, 2c (2022), 01031, 8 doi:10.1021/acs.analchem.2c01031 (međunarodna recenzija, članak, znanstveni)


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Naslov
Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus

Autori
Weitner, Tin ; Friganović, Tomislav ; Šakić, Davor

Izvornik
Analytical chemistry (0003-2700) 2c (2022); 01031, 8

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Fluorescence ; Absorption ; Optimization

Sažetak
The inner filter effect (IFE) hinders fluorescence measurements, limiting linear dependence of fluorescence signals to low sample concentrations. Modern microplate readers allow movement of the optical element in the vertical axis, changing the relative position of the focus and thus the sample geometry. The proposed Z- position IFE correction method requires only two fluorescence measurements at different known vertical axis positions (z- positions) of the optical element for the same sample. Samples of quinine sulfate, both pure and in mixtures with potassium dichromate, showed a linear dependence of corrected fluorescence on fluorophore concentration (R2 > 0.999), up to Aex ≈ 2 and Aem ≈ 0.5. The correction extended linear fluorescence response over ≈98% of the concentration range with ≈1% deviation of the calibration slope, effectively eliminating the need for sample dilution or separate absorbance measurements to account for IFE. The companion numerical IFE correction method further eliminates the need for any geometric parameters with similar results. Both methods are available online at https://ninfe.science.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA


Projekti:
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( POIROT)
EK-EFRR-KK.01.2.2.03.0006 - Centar kompetencija u molekularnoj dijagnostici (CEKOM) (Knjaz, Damir; Lauc, Gordan; Vugrek, Oliver; Polašek, Ozren, EK - KK.01.2.2.03) ( POIROT)
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Secenji, Aleksandar, EK - KK.01.1.1.01) ( POIROT)
EK-EFRR-KK.01.1.1.02.0021 - Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu (FarmInova) (Lovrić, Jasmina, EK - Ulaganje u organizacijsku reformu i infrastrukturu u sektoru istraživanja, razvoja i inovacija) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb

Profili:

Avatar Url Tin Weitner (autor)

Avatar Url Davor Šakić (autor)

Avatar Url Tomislav Friganović (autor)

Poveznice na cjeloviti tekst rada:

doi doi.org

Poveznice na istraživačke podatke:


Citiraj ovu publikaciju:

Weitner, Tin; Friganović, Tomislav; Šakić, Davor
Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus // Analytical chemistry, 2c (2022), 01031, 8 doi:10.1021/acs.analchem.2c01031 (međunarodna recenzija, članak, znanstveni)
Weitner, T., Friganović, T. & Šakić, D. (2022) Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus. Analytical chemistry, 2c, 01031, 8 doi:10.1021/acs.analchem.2c01031.
@article{article, author = {Weitner, Tin and Friganovi\'{c}, Tomislav and \v{S}aki\'{c}, Davor}, year = {2022}, pages = {8}, DOI = {10.1021/acs.analchem.2c01031}, chapter = {01031}, keywords = {Fluorescence, Absorption, Optimization}, journal = {Analytical chemistry}, doi = {10.1021/acs.analchem.2c01031}, volume = {2c}, issn = {0003-2700}, title = {Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus}, keyword = {Fluorescence, Absorption, Optimization}, chapternumber = {01031} }
@article{article, author = {Weitner, Tin and Friganovi\'{c}, Tomislav and \v{S}aki\'{c}, Davor}, year = {2022}, pages = {8}, DOI = {10.1021/acs.analchem.2c01031}, chapter = {01031}, keywords = {Fluorescence, Absorption, Optimization}, journal = {Analytical chemistry}, doi = {10.1021/acs.analchem.2c01031}, volume = {2c}, issn = {0003-2700}, title = {Inner Filter Effect Correction for Fluorescence Measurements in Microplates Using Variable Vertical Axis Focus}, keyword = {Fluorescence, Absorption, Optimization}, chapternumber = {01031} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE
  • Nature Index


Citati:





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