Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification (CROSBI ID 309323)
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Rodriguez-Temporal, David ; Alcaide, Fernando ; Mareković, Ivana ; O’Connor, James Anthony ; Gorton, Rebecca ; van Ingen, Jakko ; Van den Bossche, An ; Héry-Arnaud, Genevieve ; Beauruelle, Clémence ; Orth-Höller, Dorothea ; Palacios- Gutiérrez, Juan-José ; Tudó, Griselda ; Bou, Germán ; Ceyssens, Pieter-Jan ; Garrigó, Montserrat ; González-Martin, Julià ; Greub, Gilbert ; Hrabak, Jaroslav ; Ingebretsen, André ; Mediavilla-Gradolph, Maria Concepción ; Oviaño, Marina ; Palop, Begoña ; Pranada, Arthur B. ; Quiroga, Lidia ; Ruiz-Serrano, Maria Jesús ; Rodríguez-Sánchez, Belén
engleski
Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species- level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
MALDI-TOF MS, identification, nontuberculous mycobacteria
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Podaci o izdanju
Povezanost rada
Kliničke medicinske znanosti