engleski

PCL/Si-doped multi-phase calcium phosphate scaffolds derived from cuttlefish bone

Increasing number of studies is focused on developing biomaterials as temporary matrices for bone growth that provide a specific environment and architecture for tissue development. Calcium phosphate-based scaffolds obtained from biogenic sources are better accepted by human organism due to the presence of substituted elements (e.g. Sr2+, Mg2+, Na+, K+) that play a key role in bone regeneration. The aim of the present work was to synthesize silicon-doped biomimetic multi-phase composite scaffolds based on bioactive inorganic phases and biocompatible polymer by using simple and inexpensive methods. Porous calcium phosphate scaffolds were synthesized by hydrothermal method from cuttlefish bone, impregnated with 3-triethoxysilylpropylamine for 1‒4 times, heat-treated (1000 °C) and coated with polycaprolactone using vacuum impregnation technique. The effect of silicon doping and polymer coating on the microstructure, mechanical and biological properties of the scaffolds has been investigated. Multi-phase scaffolds based on calcium phosphates (hydroxyapatite, β-tricalcium phosphate, α-tricalcium phosphate) and calcium silicates (wollastonite, larnite, dicalcium silicate) phases were obtained. Elemental mapping revealed homogeneously dispersed silicon through the scaffolds, whereas silicon-doping increased bovine serum albumin protein adsorption. Cuttlefish bone highly porous structure was preserved leading to composite scaffolds porosity of ~78%. Compressive strength of ~1.4 MPa makes obtained composite scaffolds appropriate for non-load bearing applications. Cytocompatibility assessment by MTT assay of human mesenchymal stem cells revealed non-cytotoxicity of obtained scaffolds.

biogenic source ; biomimetic ; bone scaffold ; calcium phosphate ; calcium silicate ; silicon

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