Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1192539

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles


Pongrac, Igor M; Dobrivojević, Marina; Ahmed, Lada Brkić; Babič, Michal; Šlouf, Miroslav; Horák, Daniel; Gajović, Srećko
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles // Beilstein Journal of Nanotechnology, 7 (2016), 926-936 doi:10.3762/bjnano.7.84 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1192539 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles
(Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles)

Autori
Pongrac, Igor M ; Dobrivojević, Marina ; Ahmed, Lada Brkić ; Babič, Michal ; Šlouf, Miroslav ; Horák, Daniel ; Gajović, Srećko

Izvornik
Beilstein Journal of Nanotechnology (2190-4286) 7 (2016); 926-936

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
dextran ; maghemite ; nanoparticles ; neural stem cells ; poly(L-lysine)

Sažetak
Background: Cell tracking is a powerful tool to understand cellular migration, dynamics, homing and function of stem cell transplants. Nanoparticles represent possible stem cell tracers, but they differ in cellular uptake and side effects. Their properties can be modified by coating with different biocompatible polymers. To test if a coating polymer, poly(L-lysine), can improve the biocompatibility of nanoparticles applied to neural stem cells, poly(L-lysine)- coated maghemite nanoparticles were prepared and characterized. We evaluated their cellular uptake, the mechanism of internalization, cytotoxicity, viability and proliferation of neural stem cells, and compared them to the commercially available dextran-coated nanomag®-D-spio nanoparticles. Results: Light microscopy of Prussian blue staining revealed a concentration-dependent intracellular uptake of iron oxide in neural stem cells. The methyl thiazolyl tetrazolium assay and the calcein acetoxymethyl ester/propidium iodide assay demonstrated that poly(L-lysine)-coated maghemite nanoparticles scored better than nanomag®-D-spio in cell labeling efficiency, viability and proliferation of neural stem cells. Cytochalasine D blocked the cellular uptake of nanoparticles indicating an actin-dependent process, such as macropinocytosis, to be the internalization mechanism for both nanoparticle types. Finally, immunocytochemistry analysis of neural stem cells after treatment with poly(L- lysine)-coated maghemite and nanomag®-D-spio nanoparticles showed that they preserve their identity as neural stem cells and their potential to differentiate into all three major neural cell types (neurons, astrocytes and oligodendrocytes). Conclusion: Improved biocompatibility and efficient cell labeling makes poly(L-lysine)- coated maghemite nanoparticles appropriate candidates for future neural stem cell in vivo tracking studies.

Izvorni jezik
Hrvatski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
EK-FP7-316120 - Combining Stem Cells and Biomaterials for Brain Repair - Unlocking the Potential of the Existing Brain Research through Innovative In Vivo Molecular Imaging (GLOWBRAIN) (EK - FP7-REGPOT-2012-2013-1) ( POIROT)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Srećko Gajović (autor)

Avatar Url Lada Brkić (autor)

Avatar Url Igor Pongrac (autor)

Avatar Url Marina Radmilović (autor)

Poveznice na cjeloviti tekst rada:

doi www.beilstein-journals.org

Citiraj ovu publikaciju:

Pongrac, Igor M; Dobrivojević, Marina; Ahmed, Lada Brkić; Babič, Michal; Šlouf, Miroslav; Horák, Daniel; Gajović, Srećko
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles // Beilstein Journal of Nanotechnology, 7 (2016), 926-936 doi:10.3762/bjnano.7.84 (međunarodna recenzija, članak, znanstveni)
Pongrac, I., Dobrivojević, M., Ahmed, L., Babič, M., Šlouf, M., Horák, D. & Gajović, S. (2016) Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles. Beilstein Journal of Nanotechnology, 7, 926-936 doi:10.3762/bjnano.7.84.
@article{article, author = {Pongrac, Igor M and Dobrivojevi\'{c}, Marina and Ahmed, Lada Brki\'{c} and Babi\v{c}, Michal and \v{S}louf, Miroslav and Hor\'{a}k, Daniel and Gajovi\'{c}, Sre\'{c}ko}, year = {2016}, pages = {926-936}, DOI = {10.3762/bjnano.7.84}, keywords = {dextran, maghemite, nanoparticles, neural stem cells, poly(L-lysine)}, journal = {Beilstein Journal of Nanotechnology}, doi = {10.3762/bjnano.7.84}, volume = {7}, issn = {2190-4286}, title = {Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles}, keyword = {dextran, maghemite, nanoparticles, neural stem cells, poly(L-lysine)} }
@article{article, author = {Pongrac, Igor M and Dobrivojevi\'{c}, Marina and Ahmed, Lada Brki\'{c} and Babi\v{c}, Michal and \v{S}louf, Miroslav and Hor\'{a}k, Daniel and Gajovi\'{c}, Sre\'{c}ko}, year = {2016}, pages = {926-936}, DOI = {10.3762/bjnano.7.84}, keywords = {dextran, maghemite, nanoparticles, neural stem cells, poly(L-lysine)}, journal = {Beilstein Journal of Nanotechnology}, doi = {10.3762/bjnano.7.84}, volume = {7}, issn = {2190-4286}, title = {Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles}, keyword = {dextran, maghemite, nanoparticles, neural stem cells, poly(L-lysine)} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font