engleski

Help me out, I'm in a multistress environment!

The impact of water pollution and climate change on freshwater ecosystems has increased significantly over the decades and is a serious problem worldwide [1]. Various pollutants can co-occur and interact in different ways, crossing ecosystem boundaries and spreading to adjacent terrestrial habitats [2]. The aim of the current study was to investigate the response of non-model aquatic insects to individual and combined effects of emerging contaminants (ECs) such as pharmaceuticals and endocrine disruptors, and elevated temperatures. A laboratory microcosm experiment was conducted using a simplified freshwater food web containing bryophytes and Trichoptera larvae feeding mainly as shredders. Sampling included an initial and several successive collections that included all life stages (larvae, pupae, and the adult stage). Analyses such as total protein content, total lipid content, metabolite and lipidome profiling were performed. Preliminary results indicate that metabolome was more affected by ECs, while temperature had a greater effect on lipidome. Furthermore, increased water temperature affected phenology and resulted in earlier adult emergence. A multiple stressor effect was observed in treatments with a combination of ECs and increased water temperature, as evidenced by the greatest decrease in total protein content of larvae and pupae and total lipid content of adults. Additionally, identification of metabolites and quantification of ECs in caddisfly and moss tissues will be conducted. This research will provide valuable insights into the effects of ECs and increased water temperature on aquatic food webs. It will also provide detailed insights into the cross-ecosystem transport of ecological subsidies.

Multiple stressors ; Emerging contaminants ; Microcosm

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