engleski

Association of polymorphisms in genes encoding prothrombotic and cardiovascular risk factors with disease severity in COVID-19 patients: A pilot study

Introduction The present study aimed to assess the association of 16 polymorphisms in genes encoding prothrombotic and cardiovascular risk factors with COVID-19 disease severity: FV G1691A, FV H1299R, FII G20210A, MTHFR C677T, MTHFR A1298, factor XIII V34L, PAI-1 4G/5G, EPCR haploytpes (A1/A2/A3), eNOS -786 T>C, eNOS G894T, LTA C804A, ACE I/D, ITGB3 PIA1/A2, ITGA2B Baka/b, β-Fbg -455 G>A and ApoB R3500Q. Methods The study included 30 patients with severe COVID- 19 and 49 non-severe COVID-19 patients. All studied polymorphisms except ITGA2B Baka/b were determined using multilocus genotyping assays CVD StripAssays (ViennaLab Diagnostics, Vienna, Austria), while ITGA2B was genotyped using a real- time PCR method based on TaqMan technology. Results Higher frequency of carriers of at least one ITGB3 PIA2 allele was found in severe COVID-19 patients (P=0.009). The distribution of genotypes was significantly different for ß- Fbg -455G>A (P=0.042), with the only three homozygous AA genotypes found among severe COVID-19 patients. The association with an increased risk for severe COVID-19 was found for ITGB3, with carriers of at least one ITGB3 PIA2 allele having a 3.5-fold greater risk of severe COVID-19 (P=0.011). Genotype distribution differences were obtained for the combinations of FV H1299R and FXIII V34L (P=0.026), ITGB3 PIA1/A2 and ITGA2B Baka/b (P=0.024), and ACE I/D and PAI-1 4G/5G (P=0.046). Conclusion ITGB3 polymorphism emerged as an independent risk factor for severe COVID-19 and homozygosity for ß- Fbg -455 G>A mutation could contribute to disease severity. The combined effect of polymorphisms in genes encoding prothrombotic and cardiovascular risk factors, could further contribute to disease severity.

COVID-19 ; genetic polymorphisms ; thrombosis ; cardiovascular risk

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