Impact of positive charge and ring-size on the interactions of calixarenes with DNA, RNA and nucleotides (CROSBI ID 308525)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Krošl, Ivona, Otković, Ena ; Nikšić-Franjić, Ivana, Colasson, Benoit ; Reinaud, Olivia ; Višnjevac, Aleksandar ; Piantanida, Ivo
engleski
Impact of positive charge and ring-size on the interactions of calixarenes with DNA, RNA and nucleotides
Comparison of various calix[6]arene and calix[4]arene derivatives revealed that only analogues bearing a permanent positive charge non- covalently bind to ds-DNA and ds-RNA, by insertion into a DNA minor groove or RNA major groove. Also, these cationic analogues revealed strong and highly selective charge-dependent stabilization of AT-DNA against thermal denaturation, the calix[6]arene trication being an order of magnitude more efficient than its calix[4]arene dicationic analogue. At variance to DNA/RNA selectivity for only cationic calixarenes, both neutral and cationic calixarenes bind nucleoside monophosphates with similar efficiency, by forming tweezer-like supramolecular complexes, with nucleobases inserted between aromatic pendant arms grafted to calixarene rims. Such nucleotidecalixarene complexes were monitored by an emission change of calixarene as a function of nucleobase insertion, at variance to DNA/RNA complexes in which calixarene is inserted into the polynucleotide groove, which does not change calixarene emission – stressing the importance of the ligand insertion within a calix-basket for fluorimetric sensing.
calixarene ; ds-DNA/RNA binding ; nucleotide binding ; fluorescence ; circular dichroism
Ilustracija iz rada odabrana je za naslovnu stranicu izdanja NJC (Vol 46, No. 15) u kojem je objavljen.
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Podaci o izdanju
Povezanost rada
Biotehnologija, Interdisciplinarne prirodne znanosti, Kemija