engleski

Tailoring Algae-Based Vesicles for Drug Delivery

Traditionally used model drug delivery systems have been constructed from simple phospholipids or phospholipid membranes with anchored and embedded components, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, or exosomes. None of these systems approach the complexity of cell membranes, while the molecular mechanism of their formation is not fully understood and detection and analysis are challenging. Screening of alternative bioinspired drug delivery systems such as isolated algal membranes is limited and membrane isolation methods are not routinely used. Our goal is to identify and mechanically characterize unicellular microalgal species (1, 2) that can serve as a sustainable source for the isolation of plasma membrane vesicles (3). Using a comprehensive biophysical approach, the algal-based vesicles were characterized in terms of structure- properties and the ability to encapsulate fluorescent dyes was monitored. The observed differences in transport are attributed to differences in the structural features of the membranes and the physicochemical properties of the dye. Increased knowledge of this unique membrane system would help design marine bioinspired carriers for advanced biotechnological applications. Acknowledgments: This work is supported by the Croatian Science Foundation Projects (IP-2018-01- 5840, IP-2018-01-6910). References: 1. Pillet F et al. Bioelectrochemistry 127 (2019) 154. 2. Novosel N et al. J. Appl. Phycol. 352 (2021) 3. Ivošević DeNardis N et al. Bioelectrochemistry 134 (2020) 105724.

Algal vesicles ; loading ; molecular dyes ; permeability

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