Oleanolic acid induces cytoprotective mitophagy in HCT116 human colon carcinoma cells (CROSBI ID 716765)
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Podaci o odgovornosti
Šimić, Lidija ; Potočnjak, Iva ; Vukelić, Iva ; Batičić, Lara ; Domitrović, Robert ;
engleski
Oleanolic acid induces cytoprotective mitophagy in HCT116 human colon carcinoma cells
Colorectal cancer is a disease that occupies a very high place in terms of its malignancy and mortality. Therefore, it is of high importance to discover compounds that can efficiently ameliorate the disease and reduce mortality. Oleanolic acid (OA), a natural triterpene compound, has been shown to possess beneficial health effects, including anticancer activity. However, the effect of OA on mitophagy in colon cancer cells is unknown. The aim of this study was to investigate the mechanism and the role of autophagy/mitophagy in the anticancer activity of OA in HCT116 human colon carcinoma cells, cell line commonly used in colon cancer studies, and therapeutic drug development. Additionally, we examined chemosensitization to 5fluorouracil (5FU). OA dosedependently reduced viability of HCT116 cells, with IC50 = 29.8 µM. The expression of cleaved caspase3 and poly (ADPribose) polymerase 1 (PARP) increased by OA treatment, suggesting that OA induces apoptosis in HCT116 cells. OA induced autophagy in cancer cells by increasing expression of Beclin1, Atg5 and microtubuleassociated protein 1A/1Blight chain 3 betaII (LC3BII), which was found to be protective. Induction of mitophagy was suggested by increased expression of p62 and PTENinduced kinase 1 (PINK1) and reduced expression of translocase of outer mitochondrial membrane 20 (TOMM20), which colocalized with LC3B. OA induced nuclear accumulation of forkhead box O3a (FOXO3a) and phoshoFOXO3a. The cytotoxic activity of OA coincided by suppression of the phosphoinositide 3kinase (PI3K)/Akt and extracellular regulated kinase 1/2 (ERK1/2) pathways and activation of AMPactivated protein kinase (AMPK), cJun Nterminal kinase 1 (JNK1), and p38. The results of the study show the cytotoxic activity of OA in HCT116 human colon carcinoma cells with concomitant induction of survival autophagy and mitophagy through modulation of key signaling pathways. Moreover, OA chemosensitized HCT116 cells to 5FU.
oleanolic acid, HCT116 human colon cancer cells, apoptosis, autophagy, mitophagy, 5-Fluorouracil
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Podaci o prilogu
1-1.
2022.
objavljeno
Podaci o matičnoj publikaciji
IUBMB-FEBS-PABMB 2022 Congress 9 – 14 July 2022. Lisbon, Portugal
Podaci o skupu
IUBMB-FEBS-PABMB 2022 Congress
poster
09.07.2022-14.07.2022
Lisabon, Portugal