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Enzymatic synthesis of gold and silver nanoparticles

The usage of metal nanoparticles with exactly defined characteristics and their synthesis is among the most investigated research topics in the last decades. Gold and silver nanoparticles (NP) are considered as one of the most interesting nanoparticles due to their widespread application in health care, electronics, renewable energy and others. By their implementation in the products of the food and textile industry, many improvements in antibacterial, UV protection, flame retardant and/or hydrophobicity properties can be achieved. To overcome disadvantages during the conventional NPs synthesis, i.e. use of harsh chemicals and/or reaction conditions, enzymatic synthesis of Au- and Ag-NPs as a more convenient method was carried out in this research. For that purpose, two oxidoreductases: cellobiose dehydrogenase and glucose dehydrogenase were employed. The impact of the reaction conditions (temperature, enzyme and reactants concentration, reaction medium, pH, light and oxygen) was investigated and optimized. Successfully synthesized NPs were characterized by UV-Vis spectroscopy and transmission electron microscope (TEM). The determination of reaction kinetics and model development enabled insight in the reaction mechanism of the enzymatically catalyzed NPs.

enzyme, synthesis, gold, silver, nanoparticles

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