engleski

Recognition of DNA:RNA hybrid and triplex structure by a series of benzothiazole ligands

DNA:RNA hybrids and triplexes are formed as intermediate structures during many biologically important processes such as DNA replication, transcription, telomere replication and modulation of structure and/or function of specific genes (antigene strategy for gene regulation).[1, 2, 3, 4] Interactions of an array of nucleic acid structures with a small series of benzothiazole ligands (1-9) were screened by competition dialysis assay (Figure 1). The main aim of this study was detection of benzothiazole structures with preferential binding to DNA:RNA hybrids and ATT triplex in regard to regular (non- hybrid) DNA and RNA duplexes and singlestranded forms. Complexes of nucleic acids and benzothiazoles, screened by this method, were then characterised by UV/Vis, fluorescence, circular dichroism (CD) spectroscopy, isothermal titration calorimetry and Compounds 1 and 6 showed the highest affinities toward 13 nucleic acid structures while 5, despite lower affinities, yielded higher selectivity among studied compounds. While both 6 and 1 exerted high binding affinities toward ATT, the latter showed stronger binding to rAdT hybrid whereas the former preferred dArU hybrid.

DNA:RNA hybrids ; triplexes ; interactions ; benzothiazole ligands

nije evidentirano