engleski

Pdgfrβ Signaling inhibits BMP2-Mediated Osteogenesis in Bone Healing

Bone regeneration depends on a pool of bone/cartilage progenitor cells and mechanisms regulating differentiation. We aimed to evaluate the in vivo effects of PDGF and BMP2 signaling using bone healing models. Targeted deletion of PDGFRb in aSMA osteoprogenitors led to increased callus density and bone mass, resulting in improved biomechanical properties. To evaluate critical size bone defects, we utilized aSMACreER crossed with Ai9 reporter as a marker of osteoprogenitor cells (SMA9) and Col2.3GFP to identify mature osteoblast lineage cells. BMP2 treatment increased proportion of αSMA9+ progenitor cells, which were decreased when PDGF BB was combined with BMP2. BMP2 induced significant bone formation and a number of Col2.3GFP+ osteoblasts, which were decreased with PDGF BB. This is the first in vivo study showing inhibition of BMP2-induced osteogenesis by PDGF signaling. Controlling PDGF signaling during osteogenesis might lead to better treatment options during bone regeneration.

bone defect ; bone morphogenetic protein 2 ; Platelet derived growth factor ; osteogenesis

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