engleski

Effects of different chlorhexidine treatments on bacterial adhesion to surface of orthodontic alloys

Aim: To analyze the effect of chlorhexidine digluconate (CHX DG) treatment on the adhesion of oral bacteria to nickel-titanium (NiTi) and stainless steel (SS) alloys of orthodontic appliances. Materials and Methods: Experiment included treatment with three solutions based on CHX DG with 0.12% active substace (Perio Plus, Curasept and pure CHX DG) on four bacteria: Veilonella parvula, Agregatibacter actinomycetemcomitans, and Streptococcus oralis and mutans. The adhesive effect on orthodontic wires was read after 24 hours for S. oralis and after 72 hours for other bacteria. The minimum bactericidal concentration of the solution for each bacteria was determined by the dilution method of testing the antibacterial action. Orthodontic archwires pretreated by saliva were exposed to minimal bactericidal concentration of the solution and bacteria. Results: Commercial antiseptic products, especially Perio Plus, showed a better effect on inhibiting the adhesion of all bacteria to both alloys versus pure CHX DG solution (p <0.05). A. actinomycetemcomitans was most inhibited in adhesion out of all bacteria by CHX DG products. Greater inhibition of adherence of streptococci is seen on SS, while A. actinomycetemcomitans on NiTi. Inhibition in V. parvula is related to antiseptic product. Conclusion: Inhibition of bacterial adhesion is dependent on CHX DG, alloy and bacteria. CHX DG is effective in inhibiting bacterial adhesion to orthodontic elements and is recommended for chemical control of biofilm during orthodontic treatment with fixed appliances.

chlorhexidine ; biofilm ; adhesion ; orthodontics

Funded by Croatian Science Foundation IP-2020-02- 4027 (Environmental factors and microbial interactions in the structure of the dental biofilm).