hrvatski

Fluorimetrijsko prepoznavanje DNA i BSA ovisi o povezanosti konjugata triarilboranskog kationa s fluorescentnim supstituentom ili sekvencom peptida

Small molecule fluorescent probes targeting DNA/RNA and proteins became unavoidable tools for monitoring biological processes due to their biochemical and biomedicinal applications. Until recently boron-containing polycyclic π-systems were considered unstable in water, but in 2012 was shown that planarised triarylboranes can be stable in water and subsequently we showed that such dyes are intriguing fluorescent probes for biomacromolecules. Here, we designed and prepared two novel conjugates of triarylborane cation, differing in the type of “click” attached substituent. Both conjugates showed fluorimetric selectivity between ds-DNA (ct-DNA) and protein (BSA), whereby more flexible conjugate excelled by complete quenching emission for DNA and a strong increase for BSA.

triarilborani ; DNA/BSA probe ; fluorescencija ; CuAAC click kemija

nije evidentirano

engleski

Fluorimetric Recognition between DNA and BSA Depends on Triarylborone Catione Connectivity to Fluorescent Supstituent or Peptide Sequence

Small molecule fluorescent probes targeting DNA/RNA and proteins became unavoidable tools for monitoring biological processes due to their biochemical and biomedicinal applications. Until recently boron-containing polycyclic π-systems were considered unstable in water, but in 2012 was shown that planarised triarylboranes can be stable in water and subsequently we showed that such dyes are intriguing fluorescent probes for biomacromolecules. Here, we designed and prepared two novel conjugates of triarylborane cation, differing in the type of “click” attached substituent. Both conjugates showed fluorimetric selectivity between ds-DNA (ct-DNA) and protein (BSA), whereby more flexible conjugate excelled by complete quenching emission for DNA and a strong increase for BSA.

triarylboranes ; DNA/BSA probes ; fluorescence ; CuAAC click chemistry

nije evidentirano