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Interactions of methyl viologens of bis-(4'- ethynylpyridyl)arenes with DNA/RNA and their biological activity

The 4, 4'-bipyridyl salts (viologens), extensively investigated in the past recently again attracted more attention . Based on previous results of Marder et al on bis-(4'-ethynylpyridyl)arenes, here we studied their bis-cationic analogues. 1M and 2M showed only non-selective emission quenching upon binding to various ds-DNA/RNA, at variance to anthracene analogue, 3M, yielding an opposite fluorescence response for GC-DNA (quenching) in comparison to AT-DNA (strong increase). In the CD experiments 3M at ICD bands at λ>300 nm strongly supported intercalation, whereas 2M ICD band (330-450 nm) agreed with DNA/RNA groove binding. MTT assays and confocal microscopy showed that 3M efficiently enters the living cells, accumulating in lysosomes.

viologens ; DNA/RNA ; circular dichroism ; MTT test

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