engleski

Total phenolic and flavonoid content of methanolic extracts from Cornus mas L. and Cornus officinalis Siebold & Zucc. leaves and fruits

In this study, total phenolic and flavonoid content of methanolic extracts of leaves and fruits from six samples of Cornus mas L. and one sample of Cornus officinalis Siebold & Zucc., Cornaceae, have been investigated. Samples were air-dried, milled and extracted with methanol in an ultrasonic bath. After filtration, organic solvent was removed using a rotary evaporator and the remaining samples were suspended in distilled water, frozen and afterward subjected to lyophilization. Total phenolic content and flavonoid content were evaluated spectrophotometrically in aqueous solutions of obtained lyophilizates using the Folin-Ciocalteu method and the AlCl3 method, respectively. Pearson’s correlation coefficient (r) was used to establish the relationship between leaf and fruit total phenolic and flavonoid content (ɑ = 0.05). Data analysis was performed in Microsoft Excel (2013). Total phenolic content of C. mas leaf and fruit extracts ranged from 195.32 ± 1.59 to 311.21 ± 1.22 mg gallic acid equivalent (GAE)/g dry extract (DE) and from 9.69 ± 0.58 to 16.63 ± 0.26 mg GAE/g DE, respectively. Total phenolic contents of C. officinalis leaf and fruit extracts were 286.70 ± 1.90 mg GAE/g DE and 13.56 ± 0.15 mg GAE/g DE, respectively. Flavonoid content in leaf extracts of C. mas ranged from 1.20 ± 0.06 to 4.51 ± 0.22 mg quercetin equivalent (QE)/g DE, while flavonoid content in fruit extracts ranged from 0.18 ± 0.04 to 1.07 ± 0.02 mg QE/ g DE. Flavonoid contents of C. officinalis leaf and fruit extracts were 3.66 ± 0.03 and 0.42 ± 0.03 mg QE/g DE, respectively. Very strong negative correlation was observed between total phenolic contents in leaves and fruits of C. mas (r = -0.94, P = 0.005), while between leaf and fruit flavonoid contents of C. mas very strong positive correlation was observed (r = 0.90, P = 0.016).

Cornus mas ; Cornus officinalis ; total phenolic content ; flavonoid content

nije evidentirano