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Pregled bibliografske jedinice broj: 1180857

Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress


Habazin, Siniša; Mlinarević, Dražen; Balog, Marta; Bardak, Ana; Gaspar, Robert; Ferenc Szűcs, Kálmán; Vari, Sandor; Vučković, Frano; Lauc, Gordan; Novokmet, Mislav; Heffer, Marija
Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress // 27th Croatian meeting of chemists and chemical engineers, Book of abstracts / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna (ur.).
Zagreb: Croatian Chemical Society, 2021. str. 181-181 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 1180857 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress

Autori
Habazin, Siniša ; Mlinarević, Dražen ; Balog, Marta ; Bardak, Ana ; Gaspar, Robert ; Ferenc Szűcs, Kálmán ; Vari, Sandor ; Vučković, Frano ; Lauc, Gordan ; Novokmet, Mislav ; Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th Croatian meeting of chemists and chemical engineers, Book of abstracts / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna - Zagreb : Croatian Chemical Society, 2021, 181-181

Skup
27th Croatian meeting of chemists and chemical engineers

Mjesto i datum
Veli Lošinj, Hrvatska, 05.-08. 10. 2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Spektrometrija masa, imunoglobulin G, glikani
(Mass spectrometry, immunoglobulin G, glycans)

Sažetak
Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek,
GENOS d.o.o.

Profili:

Avatar Url Gordan Lauc (autor)

Avatar Url Marta Balog (autor)

Avatar Url Mislav Novokmet (autor)

Avatar Url Marija Heffer (autor)

Avatar Url Frano Vučković (autor)

Avatar Url Siniša Habazin (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada

Citiraj ovu publikaciju:

Habazin, Siniša; Mlinarević, Dražen; Balog, Marta; Bardak, Ana; Gaspar, Robert; Ferenc Szűcs, Kálmán; Vari, Sandor; Vučković, Frano; Lauc, Gordan; Novokmet, Mislav; Heffer, Marija
Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress // 27th Croatian meeting of chemists and chemical engineers, Book of abstracts / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna (ur.).
Zagreb: Croatian Chemical Society, 2021. str. 181-181 (poster, nije recenziran, sažetak, znanstveni)
Habazin, S., Mlinarević, D., Balog, M., Bardak, A., Gaspar, R., Ferenc Szűcs, K., Vari, S., Vučković, F., Lauc, G., Novokmet, M. & Heffer, M. (2021) Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress. U: Marković, D., Meštrović, E., Namjesnik, D. & Tomašić, V. (ur.)27th Croatian meeting of chemists and chemical engineers, Book of abstracts.
@article{article, author = {Habazin, Sini\v{s}a and Mlinarevi\'{c}, Dra\v{z}en and Balog, Marta and Bardak, Ana and Gaspar, Robert and Ferenc Sz\H{u}cs, K\'{a}lm\'{a}n and Vari, Sandor and Vu\v{c}kovi\'{c}, Frano and Lauc, Gordan and Novokmet, Mislav and Heffer, Marija}, year = {2021}, pages = {181-181}, keywords = {Spektrometrija masa, imunoglobulin G, glikani}, title = {Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress}, keyword = {Spektrometrija masa, imunoglobulin G, glikani}, publisher = {Croatian Chemical Society}, publisherplace = {Veli Lo\v{s}inj, Hrvatska} }
@article{article, author = {Habazin, Sini\v{s}a and Mlinarevi\'{c}, Dra\v{z}en and Balog, Marta and Bardak, Ana and Gaspar, Robert and Ferenc Sz\H{u}cs, K\'{a}lm\'{a}n and Vari, Sandor and Vu\v{c}kovi\'{c}, Frano and Lauc, Gordan and Novokmet, Mislav and Heffer, Marija}, year = {2021}, pages = {181-181}, keywords = {Mass spectrometry, immunoglobulin G, glycans}, title = {Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress}, keyword = {Mass spectrometry, immunoglobulin G, glycans}, publisher = {Croatian Chemical Society}, publisherplace = {Veli Lo\v{s}inj, Hrvatska} }




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