Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress (CROSBI ID 715031)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Habazin, Siniša ; Mlinarević, Dražen ; Balog, Marta ; Bardak, Ana ; Gaspar, Robert ; Ferenc Szűcs, Kálmán ; Vari, Sandor ; Vučković, Frano ; Lauc, Gordan ; Novokmet, Mislav ; Heffer, Marija
engleski
Changes in rat immunoglobulin G N-glycosylation are associated with chronic stress
Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N-glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response.
Mass spectrometry, immunoglobulin G, glycans
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Podaci o prilogu
181-181.
2021.
objavljeno
Podaci o matičnoj publikaciji
27th Croatian meeting of chemists and chemical engineers, Book of abstracts
Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna
Zagreb: Hrvatsko kemijsko društvo
2757-0754
Podaci o skupu
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)
poster
05.10.2021-08.10.2021
Veli Lošinj, Hrvatska