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Pregled bibliografske jedinice broj: 1178936

High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations


Zorić, Lara; Drinković, Nikša; Micek, Vedran; Frkanec, Leo; Türeli, Akif Emre; Günday-Türeli, Nazende; Vinković Vrček, Ivana; Frkanec, Ruža
High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations // Molecules, 27 (2022), 4; 1177, 14 doi:10.3390/molecules27041177 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1178936 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations

Autori
Zorić, Lara ; Drinković, Nikša ; Micek, Vedran ; Frkanec, Leo ; Türeli, Akif Emre ; Günday-Türeli, Nazende ; Vinković Vrček, Ivana ; Frkanec, Ruža

Izvornik
Molecules (1420-3049) 27 (2022), 4; 1177, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
High-throughput analysis ; Doxorubicin metabolites ; HPLC-FD method ; Validation ; Nanoformulation

Sažetak
Doxorubicin (DOX) is one of the most effective cytotoxic agents against malignant diseases. However, the clinical application of DOX is limited, due to dose-related toxicity. The development of DOX nanoformulations that significantly reduce its toxicity and affect the metabolic pathway of the drug requires improved methods for the quantitative determination of DOX metabolites with high specificity and sensitivity. This study aimed to develop a high-throughput method based on high-performance liquid chromatography with fluorescence detection (HPLC-FD) for the quantification of DOX and its metabolites in the urine of laboratory animals after treatment with different DOX nanoformulations. The developed method was validated by examining its specificity and selectivity, linearity, accuracy, precision, limit of detection, and limit of quantification. The DOX and its metabolites, doxorubicinol (DOXol) and doxorubicinone (DOXon), were successfully separated and quantified using idarubicin (IDA) as an internal standard (IS). The linearity was obtained over a concentration range of 0.05–1.6 μg/mL. The lowest limit of detection and limit of quantitation were obtained for DOXon at 5.0 ng/mL and 15.0 ng/mL, respectively. For each level of quality control (QC) samples, the inter- and intra-assay precision was less than 5%. The accuracy was in the range of 95.08–104.69%, indicating acceptable accuracy and precision of the developed method. The method was applied to the quantitative determination of DOX and its metabolites in the urine of rats treated by novel nanoformulated poly(lactic-co-glycolic acid) (DOX-PLGA), and compared with a commercially available DOX solution for injection (DOX-IN) and liposomal-DOX (DOX-MY).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
HRZZ-IP-2018-01-6910 - Sinteza supramolekulskih samo-udruženih nanostruktura za izgradnju naprednih funkcionalnih materijala (SUPeRNANO) (Frkanec, Leo, HRZZ - 2018-01) ( POIROT)
EK-H2020-953110 - Farmaceutski otvorena ispitna platforma za nano-farmaceutske inovativne proizvode (Phoenix) (Vinković Vrček, Ivana, EK - H2020-NMBP-TO-IND-2020-twostage) ( POIROT)
HRZZ-PZS-2019-02-4323 - Siguran pristup za razvoj nano-sustava za ciljanu isporuku lijekova u mozak (SENDER) (Vinković Vrček, Ivana, HRZZ - 2019-02) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Sveučilište u Zagrebu

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com doi.org fulir.irb.hr

Citiraj ovu publikaciju:

Zorić, Lara; Drinković, Nikša; Micek, Vedran; Frkanec, Leo; Türeli, Akif Emre; Günday-Türeli, Nazende; Vinković Vrček, Ivana; Frkanec, Ruža
High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations // Molecules, 27 (2022), 4; 1177, 14 doi:10.3390/molecules27041177 (međunarodna recenzija, članak, znanstveni)
Zorić, L., Drinković, N., Micek, V., Frkanec, L., Türeli, A., Günday-Türeli, N., Vinković Vrček, I. & Frkanec, R. (2022) High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations. Molecules, 27 (4), 1177, 14 doi:10.3390/molecules27041177.
@article{article, author = {Zori\'{c}, Lara and Drinkovi\'{c}, Nik\v{s}a and Micek, Vedran and Frkanec, Leo and T\"{u}reli, Akif Emre and G\"{u}nday-T\"{u}reli, Nazende and Vinkovi\'{c} Vr\v{c}ek, Ivana and Frkanec, Ru\v{z}a}, year = {2022}, pages = {14}, DOI = {10.3390/molecules27041177}, chapter = {1177}, keywords = {High-throughput analysis, Doxorubicin metabolites, HPLC-FD method, Validation, Nanoformulation}, journal = {Molecules}, doi = {10.3390/molecules27041177}, volume = {27}, number = {4}, issn = {1420-3049}, title = {High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations}, keyword = {High-throughput analysis, Doxorubicin metabolites, HPLC-FD method, Validation, Nanoformulation}, chapternumber = {1177} }
@article{article, author = {Zori\'{c}, Lara and Drinkovi\'{c}, Nik\v{s}a and Micek, Vedran and Frkanec, Leo and T\"{u}reli, Akif Emre and G\"{u}nday-T\"{u}reli, Nazende and Vinkovi\'{c} Vr\v{c}ek, Ivana and Frkanec, Ru\v{z}a}, year = {2022}, pages = {14}, DOI = {10.3390/molecules27041177}, chapter = {1177}, keywords = {High-throughput analysis, Doxorubicin metabolites, HPLC-FD method, Validation, Nanoformulation}, journal = {Molecules}, doi = {10.3390/molecules27041177}, volume = {27}, number = {4}, issn = {1420-3049}, title = {High-Throughput Method for the Simultaneous Determination of Doxorubicin Metabolites in Rat Urine after Treatment with Different Drug Nanoformulations}, keyword = {High-throughput analysis, Doxorubicin metabolites, HPLC-FD method, Validation, Nanoformulation}, chapternumber = {1177} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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