engleski

Effects of single-walled carbon nanotubes on the survival and release of cytokines from stretch- injured astrocytes

Introduction Astrocytes are considered to have an important role in neuronal regeneration following brain injury. Here, we explored the potential of a nanomaterial on the survival and secretory function of astrocytes after trauma. We tested the effects of single-walled carbon nanotubes (SWCNTs), chemically functionalized with poly-m- aminobenzene sulfonic acids (PABS), in an in vitro model of severe traumatic brain injury (TBI). Methods Primary mouse astrocytes were severely injured by rapid stretching. Following injury, PABS-SWCNTs were added to the cell culture medium. Injured and non-injured untreated cells were used as the control TBI and sham-treated groups, respectively. Astrocytes' survival rate within the first 24 h and the effects of PABS-SWCNTSs were determined by lactate dehydrogenase (LDH) assay. Cytokine secretion profiles were evaluated at 24 h after stretch by a multiplex array. Results Severe injury triggered an increased release of LDH from the astrocytes. Application of PABS- SWCNTs did not alter the LDH levels compared to the results from the injured, untreated cells. Cell injury caused a decrease in the Eotaxin1 and an increase in the SDF-1 alpha levels in the culture medium compared to the non-injured cells. Application of PABS-SWCNTs induced increased secretion of RANTES from the injured astrocytes, related to non-injured and injured untreated cells. Conclusion Reported results indicate that PABS-SWCNTs do not affect the survival of astrocytes subjected to severe TBI within the first 24 h. An increase in the release of RANTES from the injured cells, caused by PABS-SWCNTs addition, points to possible effects of this nanomaterial on the function of injured astrocytes. Disclosure This research was fully supported by the Croatian Science Foundation grant UIP-2017-05-9517 to KP.

astrocytes ; brain injury, traumatic ; cell death ; chemokines ; cytokines ; nanotubes, carbon

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