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Pregled bibliografske jedinice broj: 1176227

Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury


Pilipović, Kristina; Jurički, Ivana; Mladenić, Tea; Harej Hrkać, Anja; Gržeta, Nika; Parpura, Vladimir; Župan, Gordana
Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury // Neuroscience 2021
Washington, DC, USA, 2021. P348.04, 1 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1176227 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury

Autori
Pilipović, Kristina ; Jurički, Ivana ; Mladenić, Tea ; Harej Hrkać, Anja ; Gržeta, Nika ; Parpura, Vladimir ; Župan, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Neuroscience 2021 / - Washington, DC, USA, 2021

Skup
Neuroscience 2021 (SfN 2021)

Mjesto i datum
Virtualni kongres, 8-11.11.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
astrocytes ; glial fibrillary acidic protein ; inducible nitric oxide synthase ; mouse ; nanotubes, carbon ; oxidative stress ; brain injury, traumatic

Sažetak
Astrocytes are central nervous system (CNS) cells with an important role in neuronal regeneration following brain injury. The aim of this study was to investigate the effects of single-walled carbon nanotubes (SWCNTs), chemically functionalized with poly-m-aminobenzene sulfonic acids (PABS), on the oxidative protein damage and the expression of glial fibrillary acidic protein (GFAP) and the inducible nitric oxide synthase (iNOS) in astrocytes exposed to an vitro model of severe traumatic brain injury (TBI). Primary mouse astrocytes were grown on 6-well plates with deformable membranes and subjected to stretch injury, mimicking severe TBI. PABS-SWCNTs were applied to severely injured cells at 1 h post-injury. Cells were collected at 24 h following stretch and the lysates prepared for the analyses. Dot blot method was used to detect the levels of oxidatively damaged protein. Western blot analyses were used to determine the expressions of GFAP and iNOS. Non-treated cells were used as the control. Dot blot assay demonstrated that the stretch injury and the treatment with PABS- SWCNTs did not cause significant change in the levels of oxidatively damaged proteins. We observed no change in the expression of the inflammatory marker iNOS. Conversely, application of PABS- SWCNTs significantly elevated the expression level of GFAP compared to the results obtained in the samples of both control cells and the non-treated, severely stretched astrocytes. Our preliminary results suggest that the application of the investigated nanomaterial, applied as colloidal solute, to the primary astrocytes exposed to severe in vitro TBI, causes increase in the GFAP levels. This is a finding with possible relevance related to the role of GFAP in the trafficking of glutamate transporters which could be associated to the uptake of extracellular glutamate and maintenance of homeostasis in the CNS. This research was fully supported by the Croatian Science Foundation grant UIP-2017-05-9517 to KP.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-UIP-2017-05-9517 - Jednozidne ugljikove nanocjevčice u eksperimentalnoj traumatskoj ozljedi mozga (CarNanoTBI) (Pilipović, Kristina, HRZZ - 2017-05) ( POIROT)

Ustanove:
Medicinski fakultet, Rijeka


Citiraj ovu publikaciju:

Pilipović, Kristina; Jurički, Ivana; Mladenić, Tea; Harej Hrkać, Anja; Gržeta, Nika; Parpura, Vladimir; Župan, Gordana
Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury // Neuroscience 2021
Washington, DC, USA, 2021. P348.04, 1 (poster, međunarodna recenzija, sažetak, znanstveni)
Pilipović, K., Jurički, I., Mladenić, T., Harej Hrkać, A., Gržeta, N., Parpura, V. & Župan, G. (2021) Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury. U: Neuroscience 2021.
@article{article, author = {Pilipovi\'{c}, Kristina and Juri\v{c}ki, Ivana and Mladeni\'{c}, Tea and Harej Hrka\'{c}, Anja and Gr\v{z}eta, Nika and Parpura, Vladimir and \v{Z}upan, Gordana}, year = {2021}, pages = {1}, chapter = {P348.04}, keywords = {astrocytes, glial fibrillary acidic protein, inducible nitric oxide synthase, mouse, nanotubes, carbon, oxidative stress, brain injury, traumatic}, title = {Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury}, keyword = {astrocytes, glial fibrillary acidic protein, inducible nitric oxide synthase, mouse, nanotubes, carbon, oxidative stress, brain injury, traumatic}, publisherplace = {virtualni kongres}, chapternumber = {P348.04} }
@article{article, author = {Pilipovi\'{c}, Kristina and Juri\v{c}ki, Ivana and Mladeni\'{c}, Tea and Harej Hrka\'{c}, Anja and Gr\v{z}eta, Nika and Parpura, Vladimir and \v{Z}upan, Gordana}, year = {2021}, pages = {1}, chapter = {P348.04}, keywords = {astrocytes, glial fibrillary acidic protein, inducible nitric oxide synthase, mouse, nanotubes, carbon, oxidative stress, brain injury, traumatic}, title = {Chemically-functionalized single-walled carbon nanotubes increase the expression of glial fibrillary acidic protein in the mouse primary astrocytes exposed to severe in vitro traumatic brain injury}, keyword = {astrocytes, glial fibrillary acidic protein, inducible nitric oxide synthase, mouse, nanotubes, carbon, oxidative stress, brain injury, traumatic}, publisherplace = {virtualni kongres}, chapternumber = {P348.04} }




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