engleski

EVALUATION OF BIOLOGICAL ACTIVITY OF PLANT-NADES-EXTRACTS AS READY-TO-USE EXTRACT FOR FOOD AND PHARMACEUTICAL APPLICATION

Since NADES consist of simple, inexpensive and naturally occurring compounds with an implied high safety profile, it is assumed that extracts obtained by NADES may be directly used in products for human consumption without the need for expensive downstream purification steps. Considering the potential applications of NADES and NADES-based extracts, it is necessary to further evaluate their biological activity. Human cell lines are already widely used in testing biological activity of compounds present in plants and plant’s products. The application of in vitro cell cultures for this purpose is based on the fact that interaction of the test substance and biological systems occurs preferentially at the cellular level, and has an impact on the whole organism. With such approach a large number of samples, including crude extracts from screening phase, as well as pure fractions obtained during the isolation procedure, could be evaluated. Beyond that, the application of in vitro cell cultures is a good choice for preliminary screen of biological activities, which enables faster and more cost-effective studies compared to in vivo studies. The findings obtained by in vitro tests are valuable and can serve as guidelines for further required in vivo and epidemiological studies of biological activity of plants, its compounds, and products. Based on aforementioned, potential biological activity of plant polyphenolic extracts was evaluated by testing antiproliferative and antioxidant activity. In vitro cytotoxicity of prepared extracts was assessed by antiproliferation assay on two tumour cell lines, whereas for investigation of type of cell death and cell cycle arrest a flow cytometric analysis was applied. Antioxidant activity and possibility of extracts to protect cells from oxidative stress was assessed by ORAC method as well as cell-based methods.

Biological activitiy ; In vitro testing ; Natural deep eutectic solvents ; Plant extracts

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