DESIGNING A BIOCATALYTIC PROCESS INVOLVING DEEP EUTECTIC SOLVENTS: LIPASE-CATALYSED SYNTHESIS OF (R)-1- PHENYLETHANOL AS A CASE STUDY (CROSBI ID 714152)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Radović M. ; Panić M. ; Jurinjak Tušek, A. ; Cvjetko Bubalo M. ; Radošević K. ; Radojčić Redovniković I.
engleski
DESIGNING A BIOCATALYTIC PROCESS INVOLVING DEEP EUTECTIC SOLVENTS: LIPASE-CATALYSED SYNTHESIS OF (R)-1- PHENYLETHANOL AS A CASE STUDY
The synergistic use of deep eutectic solvents (DES) and biocatalyst fits logically to the efficient and sustainable production of various commercially interesting products. Namely, biocatalysis ensures catalyzing otherwise difficult transformations in high regio-, chemo- and enantioselective manner at mild and economic conditions, whereby DES can serve as strong green support for modulating/directing reaction route for obtaining the desired product. As the number of structural combinations encompassed by DES is tremendous, it is possible to design an optimal DES for each specific enzymatic reaction system. This important feature of DES allows to (i) enhance enzyme stereoselectivity, activity and stability, as well as reaction rate through enhanced substrate solubility ; (ii) enhance reaction yield (iii) enhance/modify biocatalyst stereo preference ; (iv) contribute to overall greenness of the process (including recycle and reuse). The use of DES for enzyme-catalysed reaction could be considered as “back to the roots”. Namely, when the compounds that constitute the DES are primary metabolites, namely, amino acids, organic acids, sugars or choline derivatives, they can provide cytoplasm-like natural environment for enzymes, meaning that enzymes can transform unnatural substrates in natural environment. When designing efficient biocatalytic process involving DES, independently of reaction type and enzyme used, the following steps should be included: (i) preparation and characterization of DES (ii) screening of DES for optimal enzyme performance (iii) optimization of the biocatalytic process, and (iv) downstream process design with possible scale-up. In this work green lipase-catalysed process for obtaining commercially interesting secondary alcohol (R)-1- phenylethanol of high optical purity in DES as medium was development. Several cholinium chloride-based DES were prepared and screened for optimal enzyme performance (enzyme activity, stability and enantioselectivity), followed by optimization of biocatalytic process (substrate and enyzme loading, water content in DES, reaction temperature). Recovery of the products/DES and DES recycling was successfully performed. After laboratory scale, production of (R)-1- phenylethanol was also effectively performed on a preparative scale (half liter batch reactor). The results of this study confirmed the great potential of DES in biocatalytic medium in terms of improved lipase stability and process sustainability.
Biocatalysis ; Green chemistry ; Natural deep eutectic solvents
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Podaci o prilogu
52-52.
2021.
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objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts of 1st GREENERING International Conference
Podaci o skupu
1st GREENERING International Conference
predavanje
15.02.2021-16.02.2021
ONLINE