engleski

Roles of long non-coding RNAs in regulating expression of yeast extracellular proteins

Over the past decade, advances in high-throughput RNA-sequencing methods have enabled deep insight into complexity of Saccharomyces cerevisiae transcriptome, revealing a great number of RNA transcripts, that are not translated into proteins and are therefore referred to as long non-coding RNAs (lncRNAs). In yeast, based on their sensitivity to different RNA- degradation pathways or conditions in which they are transcribed, this class of RNAs includes stable unannotated transcripts (SUTs), cryptic unstable transcripts (CUTs), meiotic unannotated transcripts (MUTs) and Xrn1-sensitive unstable transcripts (XUTs). Several classes of lncRNAs are targeted by the conserved 5’-3’ exoribonuclease Rrp6. This enzyme is associated with the exosome, a multi-subunit complex important for RNA processing and stability. Some lncRNAs are known to be implicated in various cellular processes such as gene expression, nuclear organization and chromatin structure, however vast majority of these transcripts is still unexplored. High-throughput RNA-sequencing methods discovered a plethora of antisense transcripts which are transcribed across genes encoding extracellular proteins, such as genes related to cell wall biogenesis and phosphate metabolism. We are taking advantage of high-throughput RNA-sequencing methods, as well as transcript-specific methods, to gain insight into how the cell wall transcriptome is modulated to support different life cycle phases and tolerate various environmental perturbations. Our preliminary results suggest inclusion of lncRNAs in these processes, acting via modulation of chromatin structure, promoter interference and/or overlapping sense/antisense pairs. The implications of these observations for the expression and stability for lncRNAs, mRNAs and proteins will be discussed.

yeast cell wall, Rrp6, lncRNA

nije evidentirano