Decreasing Pasteurization Treatment Efficiency against Amoeba-Grown Legionella pneumophila - Recognized Public Health Risk Factor (CROSBI ID 304618)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Knežević, Maša ; Rončević, Dobrica ; Vukić Lušić, Darija ; Mihelčić, Mirna ; Kogoj, Rok ; Keše, Darja ; Glad, Marin ; Cenov, Arijana ; Ožanič, Mateja ; Glažar Ivče, Daniela ; Šantić, Marina
engleski
Decreasing Pasteurization Treatment Efficiency against Amoeba-Grown Legionella pneumophila - Recognized Public Health Risk Factor
Legionellae are gram-negative bacteria most commonly found in freshwater ecosystems and purpose-built water systems. In humans, the bacterium causes Legionnaires’ disease (LD) or a Pontiac fever. In this study, the different waters (drinking water, pool water, cooling towers) in which Legionella pneumophila has been isolated were studied to assess the possible risk of bacterial spreading in the population. The influence of physical and chemical parameters, and interactions with Acanthamoeba castellanii on L. pneumophila, were analyzed by Heterotrophic Plate Count, the Colony-forming units (CFU) methods, transmission electron microscopy (TEM), and Sequence-Based Typing (SBT) analysis. During the study period (2013–2019), a total of 1932 water samples were analyzed, with the average annual rate of Legionella-positive water samples of 8.9%, showing an increasing trend. The largest proportion of Legionella-positive samples was found in cooling towers and rehabilitation centers (33.9% and 33.3%, respectively). Among the isolates, L. pneumophila SGs 2–14 was the most commonly identified strain (76%). The survival of Legionella was enhanced in the samples with higher pH values, while higher electrical conductivity, nitrate, and free residual chlorine concentration significantly reduced the survival of Legionella. Our results show that growth in amoeba does not affect the allelic profile, phenotype, and morphology of the bacterium but environmental L. pneumophila becomes more resistant to pasteurization treatment
Acanthamoeba ; environment ; infection ; Legionella ; water
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
19 (3)
2022.
1099
20
objavljeno
1661-7827
1660-4601
10.3390/ijerph19031099
Trošak objave rada u otvorenom pristupu
Povezanost rada
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje), Javno zdravstvo i zdravstvena zaštita, Temeljne medicinske znanosti