engleski

Effects of two types of anaesthesia on oxidative stress in plasma in patients with hydronephrosis

Human studies on oxidative stress induced by general anaesthesia are rather scarce. It is important to study effects of anaesthetics on oxidative stress, particularly in surgical procedures with high ROS production, because their effect may be pivotal for the outcome of the surgery. In the presented study, patients with hydronephrosis underwent balanced anaesthesia or Total Intravenous Anaesthesia (TIVA) with target- controlled infusion (TCI) during surgery. Blood samples from patients (N=32) were taken before induction of anaesthesia, 30 minutes and 24 hours after beginning of the surgery. Measured parameters of oxidative stress were compared between two types of anaesthesia. Malondialdehyde (MDA) was measured using HPLC with fluorescence detector. Glutathione (GSH), reactive oxygen species (ROS), total antioxidant capacity (TOC) and protein carbonyl concentration were measured spectrophotometrically. Activity of superoxide dismutase (SOD) was measured spectrophotometrically using commercial kits. Observed data were analysed by mixed modelling with oxidative stress parameter as a dependent variable and sampling time nested within an anaesthetic group as fixed effects, and subject id as a random effect. Post-hoc were performed and the false discovery rate was controlled by the Benjamini-Hochberg method. SOD activity was significantly higher in patients that underwent balanced anaesthesia than anaesthesia with TIVA/TCI 30 minutes after beginning of the surgery. There was no statistically significant difference in other measured parameters between balanced and TIVA/TCI type of anaesthesia. Results of the majority of the measured parameters of oxidative stress were significantly different 30 minutes or 24 hours after the surgery from those measured before the anaesthesia (balanced and TIVA/ TCI). Results from this study contribute to general knowledge about oxidative stress caused by anaesthesia as an important factor in causing higher rates of postoperative complications and delayed recovery after surgery.

balanced anaesthesia ; glutathione ; malondialdehyde ; surgery ; target-controlled infusion

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