engleski

The influence of lncRNAs on yeast cell wall proteins

Yeast Saccharomyces cerevisiae cell wall is an extracellular organelle important for shape, protection, and communication with the environment during growth and cell division. In the cell wall structure, mannoproteins are covalently or noncovalently attached to a polysaccharide network that usually consists of three main groups of polysaccharides: mannoproteins, β-glucan and chitin. Two types of cell wall proteins (CWPs) covalently bound to β-glucan are GPI-CWPs bound via GPI-anchors and Pir-CWPs bound via alkali labile ester bonds. The third type of proteins is noncovalently bound to β-1, 3-glucan. The cell wall is continuously remodeled as yeast cells undergo life cycle such as mating, meiosis and sporulation which are expected to be coupled with changes in the expression of genes encoding cell wall proteins. Advanced RNA sequencing methods provide insight into the yeast transcriptome and reveal pertinence of long non-coding RNAs (lncRNAs) that play important roles in various cellular processes such as transcription, translation and cell growth. Information on differences in cell wall protein levels during mitosis and meiosis is limited. We first performed relative quantification of yeast cell wall proteins in whole protein extracts using cell wall proteins, Bgl2 and Pir4. It enables us to further explore other cell wall proteins focusing on Pir5 and Spr1, which are important for sporulation. We investigated whether there is a correlation between transcription level and protein level, using the wt strain and the rrp6 mutant whose known targets are lncRNAs such as cryptic unannotated transcripts (CUTs) or meiotic unannotated transcripts (MUTs). This may indicate that sense/antisense transcripts form a double- stranded RNA (dsRNA) that may directly or indirectly prevent translation of sense mRNA.

lncRNA, Yeast, Cell wall proteins

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