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Pregled bibliografske jedinice broj: 1170182

SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish


Brnić, Dragan; Lojkić, Ivana; Škoko, Ines; Krešić, Nina; Šimić, Ivana; Keros, Tomislav; Ganjto, Marin; Štefanac, Dario; Viduka, Branka; Karšaj, Dario et al.
SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish // Environmental Research, 207 (2022), 1-10 doi:10.1016/j.envres.2021.112638 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1170182 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

Autori
Brnić, Dragan ; Lojkić, Ivana ; Škoko, Ines ; Krešić, Nina ; Šimić, Ivana ; Keros, Tomislav ; Ganjto, Marin ; Štefanac, Dario ; Viduka, Branka ; Karšaj, Dario ; Štiler, Darko ; Habrun, Boris ; Jemeršić, Lorena

Izvornik
Environmental Research (0013-9351) 207 (2022); 1-10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
SARS-CoV-2 Wastewaters Surface waters Bivalve molluscan shellfish Rotavirus A Croatia

Sažetak
The circulation of SARS-CoV-2 in the environment has been confirmed numerous times, whilst research on the bioaccumulation in bivalve molluscan shellfish (BMS) has been rather scarce. The present study aimed to fulfil the knowledge gap on SARS-CoV-2 circulation in wastewaters and surface waters in this region and to extend the current knowledge on potential presence of SARS-CoV-2 contamination in BMS. The study included 13 archive wastewater and surface water samples from the start of epidemic and 17 influents and effluents from nine wastewater treatment plants (WWTP) of different capacity and treatment stage, sampled during the second epidemic wave. From that period are the most of 77 collected BMS samples, represented by mussels, oysters and warty venus clams harvested along the Dalmatian coast. All samples were processed according to EN ISO 15216-1 2017 using Mengovirus as a whole process control. SARS-CoV-2 detection was performed by real-time and conventional RT-PCR assays targeting E, N and nsp14 protein genes complemented with nsp14 partial sequencing. Rotavirus A (RVA) real-time RT-PCR assay was implemented as an additional evaluation criterion of virus concentration techniques. The results revealed the circulation of SARS-CoV-2 in nine influents and two secondary treatment effluents from eight WWTPs, while all samples from the start of epidemic (wastewaters, surface waters) were negative which was influenced by sampling strategy. All tertiary effluents and BMS were SARS-CoV-2 negative. The results of RVA amplification were beneficial in evaluating virus concentration techniques and provided insights into RVA dynamics within the environment and community. In conclusion, the results of the present study confirm SARS-CoV-2 circulation in Croatian wastewaters during the second epidemic wave while extending the knowledge on wastewater treatment potential in SARS-CoV-2 removal. Our findings represent a significant contribution to the current state of knowledge that considers BMS of a very low food safety risk regarding SARS-CoV-2.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina



POVEZANOST RADA


Projekti:
UIP-2017-05-8580 - Rotavirusi u ekosustavu Republike Hrvatske: molekularna epidemiologija i zoonotski potencijal (REco) (Brnić, Dragan, HRZZ - 2017-05) ( POIROT)

Ustanove:
Hrvatski veterinarski institut, Zagreb

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi reader.elsevier.com

Citiraj ovu publikaciju:

Brnić, Dragan; Lojkić, Ivana; Škoko, Ines; Krešić, Nina; Šimić, Ivana; Keros, Tomislav; Ganjto, Marin; Štefanac, Dario; Viduka, Branka; Karšaj, Dario et al.
SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish // Environmental Research, 207 (2022), 1-10 doi:10.1016/j.envres.2021.112638 (međunarodna recenzija, članak, znanstveni)
Brnić, D., Lojkić, I., Škoko, I., Krešić, N., Šimić, I., Keros, T., Ganjto, M., Štefanac, D., Viduka, B. & Karšaj, D. (2022) SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish. Environmental Research, 207, 1-10 doi:10.1016/j.envres.2021.112638.
@article{article, author = {Brni\'{c}, Dragan and Lojki\'{c}, Ivana and \v{S}koko, Ines and Kre\v{s}i\'{c}, Nina and \v{S}imi\'{c}, Ivana and Keros, Tomislav and Ganjto, Marin and \v{S}tefanac, Dario and Viduka, Branka and Kar\v{s}aj, Dario and \v{S}tiler, Darko and Habrun, Boris and Jemer\v{s}i\'{c}, Lorena}, year = {2022}, pages = {1-10}, DOI = {10.1016/j.envres.2021.112638}, keywords = {SARS-CoV-2 Wastewaters Surface waters Bivalve molluscan shellfish Rotavirus A Croatia}, journal = {Environmental Research}, doi = {10.1016/j.envres.2021.112638}, volume = {207}, issn = {0013-9351}, title = {SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish}, keyword = {SARS-CoV-2 Wastewaters Surface waters Bivalve molluscan shellfish Rotavirus A Croatia} }
@article{article, author = {Brni\'{c}, Dragan and Lojki\'{c}, Ivana and \v{S}koko, Ines and Kre\v{s}i\'{c}, Nina and \v{S}imi\'{c}, Ivana and Keros, Tomislav and Ganjto, Marin and \v{S}tefanac, Dario and Viduka, Branka and Kar\v{s}aj, Dario and \v{S}tiler, Darko and Habrun, Boris and Jemer\v{s}i\'{c}, Lorena}, year = {2022}, pages = {1-10}, DOI = {10.1016/j.envres.2021.112638}, keywords = {SARS-CoV-2 Wastewaters Surface waters Bivalve molluscan shellfish Rotavirus A Croatia}, journal = {Environmental Research}, doi = {10.1016/j.envres.2021.112638}, volume = {207}, issn = {0013-9351}, title = {SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish}, keyword = {SARS-CoV-2 Wastewaters Surface waters Bivalve molluscan shellfish Rotavirus A Croatia} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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