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TLC and numerical methods in the investigation of flavonoids and of phenolic acids of Salviae folium

A series of simple mathematical techniques for the eavluation of solvents and solvent combinations in TLC have been investigated. The methods have been applied to an Rf data set of flavonoids and phenolic acids identified in the methanolic extract of Salviae folium. The most suitable TLC systems for the separation of investigated compounds are: ethyl acetate-formic acids-acetic acid-water ( 100:11:11:27 ) and ethyl acetate-formic acid-water ( 8:1:1 ).

Salviae folium; flavonoids; phenolic acids; numerical methods; TLC

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