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SELECTIVE GENOTOXICITY OF HALOGENATED BOROXINE, K2(B3O3F4OH) IN UT-7 LEUKEMIA CELL LINE AND NORMAL PERIPHERAL BLOOD MONONUCLEAR CELLS

Halogenated boroxine (HB), K2(B3O3F4OH), is a synthetic derivative of boronic acid with previously proven bioactive potential in different in vitro and in vivo model-systems. Its effects in hematological malignancies have not been tested yet. In this work, we aimed to evaluate the genotoxic potential of HB using alkaline comet assay in human acute myeloid leukemia cell line UT-7 (ACC 137) and normal peripheral blood mononuclear cells (PBMCs), respectively. UT-7 leukemia cells and normal PBMCs isolated from healthy donor were incubated at 37° C for the cultivation period of 72 h. Cultures were treated with HB in concentrations of 0.1, 0.2 and 0.4 mg/mL. Negative and positive controls were set up as well. Cell damage was analyzed using Comet Assay IV software (Instem, UK) and measuring tail intensity (%). Obtained results showed significant increase in tail intensity (p<0.001) in all HB treatments of UT-7 cells when compared to negative control. The frequency of hedgehog cells was registered in HB treatments of UT-7 cells for 0.2 mg/mL (49.5%) and 0.4 mg/mL (83.3%), indicating high dose-dependent genotoxic damage. In PBMCs, tail intensity was significantly increased (p<0.001) only in the highest tested concentration of HB in comparison to negative control. Hedgehog cells were not found in HB treated PBMCs. These results revealed selective genotoxicity of HB in tumor compared to normal cells in vitro.

comet assay, genotoxic damage, UT-7 cell line, PBMCs

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