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Pregled bibliografske jedinice broj: 1167157

The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity


Tadijan, Ana; Humphries, Jonathan D.; Samaržija, Ivana; Stojanović, Nikolina; Zha, Junzhe; Čuljak, Kristina; Tomić, Marija; Paradžik, Mladen; Nestić, Davor; Kang, Heemin et al.
The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity // Frontiers in cell and developmental biology, 9 (2021), 786758, 17 doi:10.3389/fcell.2021.786758 (međunarodna recenzija, članak, znanstveni)


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Naslov
The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity

Autori
Tadijan, Ana ; Humphries, Jonathan D. ; Samaržija, Ivana ; Stojanović, Nikolina ; Zha, Junzhe ; Čuljak, Kristina ; Tomić, Marija ; Paradžik, Mladen ; Nestić, Davor ; Kang, Heemin ; Humphries, Martin J. ; Ambriović-Ristov, Andreja

Izvornik
Frontiers in cell and developmental biology (2296-634X) 9 (2021); 786758, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adhesome ; hemidesmosome ; integrin alpha 6 beta 4 ; laminin-332 ; integrin alpha v beta 3 ; integrin crosstalk ; anticancer drug sensitivity ; keratins 5/14

Sažetak
Integrins are heterodimeric cell surface glycoproteins used by cells to bind to the extracellular matrix (ECM) and regulate tumor cell proliferation, migration and survival. A causative relationship between integrin expression and resistance to anticancer drugs has been demonstrated in different tumors, including head and neck squamous cell carcinoma. Using a Cal27 tongue squamous cell carcinoma model, we have previously demonstrated that de novo expression of integrin αVβ3 confers resistance to several anticancer drugs (cisplatin, mitomycin C and doxorubicin) through a mechanism involving downregulation of active Src, increased cell migration and invasion. In the integrin αVβ3 expressing Cal27-derived cell clone 2B1, αVβ5 expression was also increased, but unrelated to drug resistance. To identify the integrin adhesion complex (IAC) components that contribute to the changes in Cal27 and 2B1 cell adhesion and anticancer drug resistance, we isolated IACs from both cell lines. Mass spectrometry (MS)-based proteomics analysis indicated that both cell lines preferentially, but not exclusively, use integrin α6β4, which is classically found in hemidesmosomes. The anticancer drug resistant cell clone 2B1 demonstrated an increased level of α6β4 accompanied with increased deposition of a laminin-332-containing ECM. Immunofluorescence and electron microscopy demonstrated the formation of type II hemidesmosomes by both cell types. Furthermore, suppression of α6β4 expression in both lines conferred resistance to anticancer drugs through a mechanism independent of αVβ3, which implies that the cell clone 2B1 would have been even more resistant had the upregulation of α6β4 not occurred. Taken together, our results identify a key role for α6β4- containing type II hemidesmosomes in regulating anticancer drug sensitivity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2019-04-1577 - Integrin alpha V beta 5-povezane fokalne i retikularne adhezije u melanomu (AdMeFoRe) (Ambriović Ristov, Andreja, HRZZ - 2019-04) ( POIROT)
IP-2013-11-2465 - Molekularni mehanizmi povećanja osjetljivosti na protutumorske lijekove stanica karcinoma dojke i melanoma čovjeka utišavanjem integrina (INSILCELL) (Ambriović Ristov, Andreja, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi www.frontiersin.org doi.org fulir.irb.hr

Citiraj ovu publikaciju:

Tadijan, Ana; Humphries, Jonathan D.; Samaržija, Ivana; Stojanović, Nikolina; Zha, Junzhe; Čuljak, Kristina; Tomić, Marija; Paradžik, Mladen; Nestić, Davor; Kang, Heemin et al.
The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity // Frontiers in cell and developmental biology, 9 (2021), 786758, 17 doi:10.3389/fcell.2021.786758 (međunarodna recenzija, članak, znanstveni)
Tadijan, A., Humphries, J., Samaržija, I., Stojanović, N., Zha, J., Čuljak, K., Tomić, M., Paradžik, M., Nestić, D. & Kang, H. (2021) The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity. Frontiers in cell and developmental biology, 9, 786758, 17 doi:10.3389/fcell.2021.786758.
@article{article, author = {Tadijan, Ana and Humphries, Jonathan D. and Samar\v{z}ija, Ivana and Stojanovi\'{c}, Nikolina and Zha, Junzhe and \v{C}uljak, Kristina and Tomi\'{c}, Marija and Parad\v{z}ik, Mladen and Nesti\'{c}, Davor and Kang, Heemin and Humphries, Martin J. and Ambriovi\'{c}-Ristov, Andreja}, year = {2021}, pages = {17}, DOI = {10.3389/fcell.2021.786758}, chapter = {786758}, keywords = {adhesome, hemidesmosome, integrin alpha 6 beta 4, laminin-332, integrin alpha v beta 3, integrin crosstalk, anticancer drug sensitivity, keratins 5/14}, journal = {Frontiers in cell and developmental biology}, doi = {10.3389/fcell.2021.786758}, volume = {9}, issn = {2296-634X}, title = {The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity}, keyword = {adhesome, hemidesmosome, integrin alpha 6 beta 4, laminin-332, integrin alpha v beta 3, integrin crosstalk, anticancer drug sensitivity, keratins 5/14}, chapternumber = {786758} }
@article{article, author = {Tadijan, Ana and Humphries, Jonathan D. and Samar\v{z}ija, Ivana and Stojanovi\'{c}, Nikolina and Zha, Junzhe and \v{C}uljak, Kristina and Tomi\'{c}, Marija and Parad\v{z}ik, Mladen and Nesti\'{c}, Davor and Kang, Heemin and Humphries, Martin J. and Ambriovi\'{c}-Ristov, Andreja}, year = {2021}, pages = {17}, DOI = {10.3389/fcell.2021.786758}, chapter = {786758}, keywords = {adhesome, hemidesmosome, integrin alpha 6 beta 4, laminin-332, integrin alpha v beta 3, integrin crosstalk, anticancer drug sensitivity, keratins 5/14}, journal = {Frontiers in cell and developmental biology}, doi = {10.3389/fcell.2021.786758}, volume = {9}, issn = {2296-634X}, title = {The Tongue Squamous Carcinoma Cell Line Cal27 Primarily Employs Integrin α6β4-Containing Type II Hemidesmosomes for Adhesion Which Contribute to Anticancer Drug Sensitivity}, keyword = {adhesome, hemidesmosome, integrin alpha 6 beta 4, laminin-332, integrin alpha v beta 3, integrin crosstalk, anticancer drug sensitivity, keratins 5/14}, chapternumber = {786758} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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