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Pregled bibliografske jedinice broj: 1166920

Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes.


Bertović, Ivana; Bura, Ana; Jurak Begonja, Antonija
Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes. // Platelets, 12 (2021), 1-13 doi:10.1080/09537104.2021.2007869 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1166920 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes.
(Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes)

Autori
Bertović, Ivana ; Bura, Ana ; Jurak Begonja, Antonija

Izvornik
Platelets (0953-7104) 12 (2021); 1-13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
megakarocytes, bone marrow, fetal liver

Sažetak
Multiple lines of evidence support differences in the megakaryopoiesis during development. Murine in vitro models to study megakaryopoiesis employ cultured megakaryocytes MKs derived from adult bone marrow (BM) or fetal livers (FL) of mouse embryos. Mouse models allow to study the molecular basis for cellular changes utilizing conditional or knock-out models and permit further in vitro genetic or pharmacological manipulations. Despite being extensively used, MKs cultured from these two sources have not been systematically compared. In the present study, we compared BM- and FL- derived MKs, assessing their size, proplatelet production capacity, expression of common MK markers (αIIb, β3, GPIb α, β) and cytoskeletal proteins (filamin A, β1-tubulin, actin), the subcellular appearance of α-granules (VWF), membranes (GPIbβ) and cytoskeleton (F-actin) throughout in vitro development. We demonstrate that FL MKs although smaller in size, spontaneously produce more proplatelets than BM MKs and at earlier stages express more β1-tubulin. In addition, early FL MKs show increased internal GPIbβ staining and present higher GPIbβ (early and late) and VWF (late stages) total fluorescence intensity (TFI)/cell size than BM MKs. BM MKs have up-regulated TPO signaling corresponding to their bigger size and ploidy, without changes in c-Mpl. Expressing endogenous β1-tubulin or the presence of heparin improves BM MKs ability to produce proplatelets. These data suggest that FL MKs undergo cytoplasmic maturation earlier than BM MKs and that this, in addition to higher β1-tubulin levels and GPIb, supported with an extensive F- actin network, could contribute to more efficient proplatelet formation in vitro.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
Ostalo-ASH-2019 - Nucleolar control of megakaryopoiesis (NucMeg) (Jurak Begonja, Antonija, Ostalo - American Society of Hematology (ASH) Global Research Award) ( POIROT)
HRZZ-UIP-2014-09-2400 - Uloga fosfoinozitida u nastanku trombocita (MkPI) (Jurak Begonja, Antonija, HRZZ - 2014-09) ( POIROT)
Ostalo-CRP/HRV15-04_EC - Deciphering the role of phosphoinositol lipids in thrombopoiesis (Jurak Begonja, Antonija, Ostalo - CRP-ICGEB Early Career Return Grant) ( POIROT)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-188-1343 - Identifikacija novih interakcijskih partnera Vps34 u megakariopoezi (Jurak Begonja, Antonija, NadSve - UNIRI projekti 2018) ( POIROT)

Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju

Profili:

Avatar Url Ana Bura (autor)

Avatar Url Ivana Bertović (autor)

Avatar Url Antonija Jurak Begonja (autor)

Poveznice na cjeloviti tekst rada:

doi www.tandfonline.com

Citiraj ovu publikaciju:

Bertović, Ivana; Bura, Ana; Jurak Begonja, Antonija
Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes. // Platelets, 12 (2021), 1-13 doi:10.1080/09537104.2021.2007869 (međunarodna recenzija, članak, znanstveni)
Bertović, I., Bura, A. & Jurak Begonja, A. (2021) Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes.. Platelets, 12, 1-13 doi:10.1080/09537104.2021.2007869.
@article{article, author = {Bertovi\'{c}, Ivana and Bura, Ana and Jurak Begonja, Antonija}, year = {2021}, pages = {1-13}, DOI = {10.1080/09537104.2021.2007869}, keywords = {megakarocytes, bone marrow, fetal liver}, journal = {Platelets}, doi = {10.1080/09537104.2021.2007869}, volume = {12}, issn = {0953-7104}, title = {Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes.}, keyword = {megakarocytes, bone marrow, fetal liver} }
@article{article, author = {Bertovi\'{c}, Ivana and Bura, Ana and Jurak Begonja, Antonija}, year = {2021}, pages = {1-13}, DOI = {10.1080/09537104.2021.2007869}, keywords = {megakarocytes, bone marrow, fetal liver}, journal = {Platelets}, doi = {10.1080/09537104.2021.2007869}, volume = {12}, issn = {0953-7104}, title = {Developmental differences of in vitro cultured murine bone marrow- and fetal liver-derived megakaryocytes}, keyword = {megakarocytes, bone marrow, fetal liver} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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