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izvor podataka: crosbi

Comparative analysis of biological and functional properties of bone marrow mesenchymal stromal cells expanded in media with different platelet lysate content (CROSBI ID 302497)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Škifić, Marijana ; Golemović, Mirna ; Crkvenac- Gornik, Kristina ; Vrhovac, Radovan ; Golubić Čepulić, Branka Comparative analysis of biological and functional properties of bone marrow mesenchymal stromal cells expanded in media with different platelet lysate content // Cells tissues organs, 205 (2018), 226-239

Podaci o odgovornosti

Škifić, Marijana ; Golemović, Mirna ; Crkvenac- Gornik, Kristina ; Vrhovac, Radovan ; Golubić Čepulić, Branka

engleski

Comparative analysis of biological and functional properties of bone marrow mesenchymal stromal cells expanded in media with different platelet lysate content

Due to their ability to induce immunological tolerance in the recipient, mesenchymal stromal cells (MSCs) have been utilized in the treatment of various hematological and immune- and inflammation-mediated diseases. The clinical application of MSCs implies prior in vitro expansion that usually includes the use of fetal bovine serum (FBS). The present study evaluated the effect of different platelet lysate (PL) media content on the biological properties of MSCs. MSCs were isolated from the bone marrow of 13 healthy individuals and subsequently expanded in three different culture conditions (10% PL, 5% PL, 10% FBS) during 4 passages. The cells cultured in different conditions had comparable immunophenotype, clonogenic potential, and differentiation capacity. However, MSC growth was significantly enhanced in the presence of PL. Cultures supplemented with 10% PL had a higher number of cumulative population doublings in all passages when compared to the 5% PL condition (p < 0.03). Such a difference was also observed when 10% PL and 10% FBS conditions were compared (p < 0.005). A statistically significant difference in population doubling time was determined only between the 10% PL and 10% FBS conditions (p < 0.005). Furthermore, MSCs cultured in 10% PL were able to cause a 66.9% reduction of mitogen-induced lymphocyte proliferation. Three chromosome aberrations were detected in PL conditions. Since two changes occurred in the same donor, it is possible they were donor dependent rather than caused by the culture condition. These findings demonstrate that a 10% PL condition enables a higher yield of MSCs within a shorter time without altering MSC properties, and should be favored over the 5% PL condition.

Mesenchymal stromal cells ; Platelet lysate ; Fetal bovine serum ; In vitro expansion ; Chromosome aberrations

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Podaci o izdanju

205

2018.

226-239

objavljeno

1422-6405

1422-6421

Povezanost rada

Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

Indeksiranost