Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1164834

NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix


Proust, Bastien; Radić, Martina; Škrobot Vidaček, Nikolina; Cottet, Cécile; Attia, Stéphane; Lamarche, Frédéric; Ačkar, Lucija; Godinić Mikulčić, Vlatka; Tokarska-Schlattner, Malgorzata; Ćetković, Helena et al.
NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix // Cell and Bioscience, 11 (2021), 1; 195, 23 doi:10.1186/s13578-021-00707-0 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1164834 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix

Autori
Proust, Bastien ; Radić, Martina ; Škrobot Vidaček, Nikolina ; Cottet, Cécile ; Attia, Stéphane ; Lamarche, Frédéric ; Ačkar, Lucija ; Godinić Mikulčić, Vlatka ; Tokarska-Schlattner, Malgorzata ; Ćetković, Helena ; Schlattner, Uwe ; Herak Bosnar, Maja

Izvornik
Cell and Bioscience (2045-3701) 11 (2021), 1; 195, 23

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NDP kinase ; NME ; nm23 ; Mitochondria ; RCC1L ; WBSCR16

Sažetak
Background NME6 is a member of the nucleoside diphosphate kinase (NDPK/NME/Nm23) family which has key roles in nucleotide homeostasis, signal transduction, membrane remodeling and metastasis suppression. The well-studied NME1-NME4 proteins are hexameric and catalyze, via a phospho- histidine intermediate, the transfer of the terminal phosphate from (d)NTPs to (d)NDPs (NDP kinase) or proteins (protein histidine kinase). For the NME6, a gene/protein that emerged early in eukaryotic evolution, only scarce and partially inconsistent data are available. Here we aim to clarify and extend our knowledge on the human NME6. Results We show that NME6 is mostly expressed as a 186 amino acid protein, but that a second albeit much less abundant isoform exists. The recombinant NME6 remains monomeric, and does not assemble into homo-oligomers or hetero- oligomers with NME1-NME4. Consequently, NME6 is unable to catalyze phosphotransfer: it does not generate the phospho-histidine intermediate, and no NDPK activity can be detected. In cells, we could resolve and extend existing contradictory reports by localizing NME6 within mitochondria, largely associated with the mitochondrial inner membrane and matrix space. Overexpressing NME6 reduces ADP-stimulated mitochondrial respiration and complex III abundance, thus linking NME6 to dysfunctional oxidative phosphorylation. However, it did not alter mitochondrial membrane potential, mass, or network characteristics. Our screen for NME6 protein partners revealed its association with NME4 and OPA1, but a direct interaction was observed only with RCC1L, a protein involved in mitochondrial ribosome assembly and mitochondrial translation, and identified as essential for oxidative phosphorylation. Conclusions NME6, RCC1L and mitoribosomes localize together at the inner membrane/matrix space where NME6, in concert with RCC1L, may be involved in regulation of the mitochondrial translation of essential oxidative phosphorylation subunits. Our findings suggest new functions for NME6, independent of the classical phosphotransfer activity associated with NME proteins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-4021 - Struktura, funkcija i evolucija proteina Nme6/Nm23-H6 (Nemo6) (Herak-Bosnar, Maja, HRZZ - 2016-06) ( POIROT)
HRZZ-IP-2019-04-5382 - Geni spužvi povezani s nastankom raka (CanSponGE) (Ćetković, Helena, HRZZ - 2019-04) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi cellandbioscience.biomedcentral.com fulir.irb.hr

Citiraj ovu publikaciju:

Proust, Bastien; Radić, Martina; Škrobot Vidaček, Nikolina; Cottet, Cécile; Attia, Stéphane; Lamarche, Frédéric; Ačkar, Lucija; Godinić Mikulčić, Vlatka; Tokarska-Schlattner, Malgorzata; Ćetković, Helena et al.
NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix // Cell and Bioscience, 11 (2021), 1; 195, 23 doi:10.1186/s13578-021-00707-0 (međunarodna recenzija, članak, znanstveni)
Proust, B., Radić, M., Škrobot Vidaček, N., Cottet, C., Attia, S., Lamarche, F., Ačkar, L., Godinić Mikulčić, V., Tokarska-Schlattner, M. & Ćetković, H. (2021) NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix. Cell and Bioscience, 11 (1), 195, 23 doi:10.1186/s13578-021-00707-0.
@article{article, author = {Proust, Bastien and Radi\'{c}, Martina and \v{S}krobot Vida\v{c}ek, Nikolina and Cottet, C\'{e}cile and Attia, St\'{e}phane and Lamarche, Fr\'{e}d\'{e}ric and A\v{c}kar, Lucija and Godini\'{c} Mikul\v{c}i\'{c}, Vlatka and Tokarska-Schlattner, Malgorzata and \'{C}etkovi\'{c}, Helena and Schlattner, Uwe and Herak Bosnar, Maja}, year = {2021}, pages = {23}, DOI = {10.1186/s13578-021-00707-0}, chapter = {195}, keywords = {NDP kinase, NME, nm23, Mitochondria, RCC1L, WBSCR16}, journal = {Cell and Bioscience}, doi = {10.1186/s13578-021-00707-0}, volume = {11}, number = {1}, issn = {2045-3701}, title = {NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix}, keyword = {NDP kinase, NME, nm23, Mitochondria, RCC1L, WBSCR16}, chapternumber = {195} }
@article{article, author = {Proust, Bastien and Radi\'{c}, Martina and \v{S}krobot Vida\v{c}ek, Nikolina and Cottet, C\'{e}cile and Attia, St\'{e}phane and Lamarche, Fr\'{e}d\'{e}ric and A\v{c}kar, Lucija and Godini\'{c} Mikul\v{c}i\'{c}, Vlatka and Tokarska-Schlattner, Malgorzata and \'{C}etkovi\'{c}, Helena and Schlattner, Uwe and Herak Bosnar, Maja}, year = {2021}, pages = {23}, DOI = {10.1186/s13578-021-00707-0}, chapter = {195}, keywords = {NDP kinase, NME, nm23, Mitochondria, RCC1L, WBSCR16}, journal = {Cell and Bioscience}, doi = {10.1186/s13578-021-00707-0}, volume = {11}, number = {1}, issn = {2045-3701}, title = {NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix}, keyword = {NDP kinase, NME, nm23, Mitochondria, RCC1L, WBSCR16}, chapternumber = {195} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font