engleski

Analysis of sulfur-containing volatile compounds from two wild-growing Brassicaceae plants by HS-SPME/GC-MS

The present study represents the continuation of our research on chemistry of volatile compounds from wild-growing Brassicaceae plants 1-5. The organic compounds typically presented in plants of Brassicaceae family are glucosinolates. While glucosinolate are nonvolatile compounds, their decomposition products are volatile mostly sulfur-containing compounds. This study is focused on extraction of volatile compounds of two plants, Capsella rubella and Cardaria draba, using headspace solid phase microextraction (HS-SPME), and their analysis by gas chromatography-mass spectrometry (GC-MS). HS-SPME is a simple, fast, relatively inexpensive and solvent-free technique which is a widely used for the extraction of volatile compounds from fruits, vegetables and flowers while the GC-MS is the main analytical method for the analysis of volatile profiles of various origin. Capsella rubella and Cardaria draba are wild-growing Brassicaceae plants widespread in mediterranean part of Croatia. They grow on open places exposed to sunlight such as fields, pastures, meadows, waste places, and along roadsides. The volatile sulfur-containing compounds have not been so commonly examined in these two plants 1, 2, especially not using above mentioned HS-SPME coupled with GC-MS. Results presented in this study revealed that sulfur-containing compounds were qualitatively and quantiatively dominating constituents in headspaces of investigated plants. Thus, C. rubella headspaces were characterized by a high percentage of allyl isothiocyanate (79.3 and 67.2%) followed by allyl thiocyanate (11.0 and 8.8%). Dominating compound in headspaces of Cardaria draba was 4-(methylthio)butyl-isothiocyanate (91.4 and 94.2%). Considering the most abundant compounds in headspaces of investigated plants, it can be concluded that the main glucosinolate of Capsella rubella is sinigrin and of Cardaria draba is glucoerucin.

Capsella rubella, Cardaria draba, volatiles, HS-SPME/GC-MS

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