engleski

Comparative study of human and experimental mouse teratocarcinoma

Teratocarcinoma (TCa) is a type of mixed testicular germ cell tumor (TGCT) composed of teratoma and embryonal carcinoma. In 1970s Solter, Damjanov and Škreb discovered that TCa could be produced by transplanting gastrulating mouse embryos underneath a kidney capsule of a syngeneic animal. TCa was identified by classical histological analysis and now this experimental mouse teratocarcinoma model is considered as suitable for studying TGCT biology. Molecular systematic analysis and comparison of human and mouse model TCa have not been performed. The purpose of this research is to compare proliferative and apoptotic activity in human and mouse experimental TCa. For immunohistochemical detection of PCNA and Caspase-3 expression, 20 human testicular teratocarcinoma and 14 experimental mouse teratocarcinoma paraffin- embedded tissues were used. Slides were semi- quantitatively analyzed and results were categorised regarding percentage of IHC-positively stained cells as 0 (0%), 1 (<10%), 2 (10-50%) and 3 (>50%). Differences in rates of proliferation and apoptosis between human and mouse TCa were statistically significant. Most of experimental mouse TCa (64%) and just 30% human TCa showed >50% PCNApositively stained cells. With respect to Caspase-3 staining, mouse TCa samples were similarly distributed among categories 1, 2 and 3 while 70% of human TCa belonged to category 1. Analysis of proliferation and apoptosis based on percentage of PCNA and Caspase-3 positively stained cells statistically significant differ between human and mouse experimental TCa. This result may be due to small and unequal number of samples. Further analysis should be done and we are planing to conduct Western blot analysis of PCNA and Caspase-3. Acknowledgments: This study was supported by Scientific Center of Excellence for Reproductive and Regenerative Medicine, Republic of Croatia, and by the European Union through the European Regional Development Fund, under grant agreement No. KK.01.1.1.01.0008, project „Reproductive and Regenerative Medicine - Exploring New Platforms and Potentials.

teratocarcinoma ; PCNA ; caspase-3

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