engleski

Proliferative and apoptotic activity in human and mouse teratocarcinoma

Testicular germ cell tumors (TGCT) are the most common type of malignancies in young men. Although it has been reported that initiation of TGCT occurs in utero, the molecular mechanisms of TGCTs etiology still need to be elucidated. Teratocarcinoma (TCa) is a type of TGCT composed of teratoma and embryonal carcinoma. It can be induced experimentaly by transplanting gastrulating mouse embryos underneath a kidney capsule of a syngeneic animal. The aim of this research is to compare proliferative and apoptotic activity in human and mouse TCa on molecular level. Overall, 20 human testicular teratocarcinoma and 14 experimental mouse teratocarcinoma, paraffin- embedded tissues were used for IHC staining with antibodies to PCNA and CASPASE-3. Slides were semi-quantitatively analyzed and results were categorised regarding percentage of IHC-positively stained cells as 0 (0%), 1 (<10%), 2 (10-50%) and 3 (>50%). Proliferation and apoptosis between human and mouse TCa differ with statistical significans. Regarding proliferative activity detected by PCNA staining, 64% of experimental mouse TCa and just 30% human TCa were classified into category 3. With respect to CASPASE-3 staining, mouse TCa samples were equally distributed among categories 1, 2, and 3 while 70% of human TCa belonged to category 1. Immunohistochemical analysis of proliferation and apoptosis analysed with PCNA and CASPASE-3, showed statistically significant difference between human and mouse TCa. This result may be due to small and unequal number of samples hence extending sample size is planned, as well as PCNA and CASPASE-3 protein expression analysis by capillary Western immunoassay.

teratocarcinoma ; proliferation

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