engleski

Comparison of teratoma components between human testicular germ cell tumors and the experimental mouse teratocarcinoma model

Testicular germ cell tumors are the most frequent malignancies of the young male population frequently containing a teratoma component. A teratocarcinoma type of the non-seminomatous mammalian tumor is modeled by isotransplantation of the 7.5 days old mouse egg cylinder beneath the kidney capsule of the adult mouse. Our aim was to analyze in more detail components of teratoma in this model by comparison of the tissue types and cell proliferation potential with those assessed in the human testicular tumors containing a teratoma component. In total, thirty four experimental C3H mouse (4-weeks-old), ten experimental C3H mouse (8-weeks-old) tumors and thirty four human testicular tumors with a teratoma component were analysed. Classical hematoxylin and eosin histology and immunohistochemisty with primary antibodies against proliferation marker (PCNA) were performed. Statistical analysis was performed by the Fisher's exact test and Student's t-test. In the mouse model that was growing for 4-weeks in the isotransplant, teratomatous components which were significanty more frequent than in human tumors were: stratified squamous epithelium (P=0.0149), glia (P=0.0001), neural tubes (P=0.0089), skeletal muscle (P=0.0037), white adipose tissue (P=0.048), and columnar epithelium (P=0.0415). Connective tissue (loose and dense) (P=0.02808) was significanty more frequent in the human teratoma component. Also, PCNA expression was higher in the teratoma of the mouse model in 4-weeks old isotransplants than in human tumors. This high capacity for cell proliferation in mouse tumors leads also to a 13 times higher mass of 8- weeks old tumors in comparison to those grown for 4 weeks, only (P=0.0005). Our results suggest a difference in tissue composition and cellular proliferation of the teratomatous component between the mouse teratocarcinoma model and human non seminomatous germ cell tumors with a teratomatous component

testicular germ cell tumors ; teratoma components ; mouse teratocarcinoma model

nije evidentirano