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Pregled bibliografske jedinice broj: 1162835

HDACi drugs hamper experimental mouse teratoma development


Krasi, Jure; Škara, Lucija; Katušić Bojanac, Ana; Bulic Jakus, Floriana; Ježek, Davor; Ulamec, Monika; Sinčić, Nino
HDACi drugs hamper experimental mouse teratoma development // Andrology 2020 12th International/11th European/ 32nd German Congress of Andrology 5–9 December 2020 DIGITAL Abstracts
Gablitz: Krause & Pachernegg GmbH, 2020. str. 32-33 (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
HDACi drugs hamper experimental mouse teratoma development

Autori
Krasi, Jure ; Škara, Lucija ; Katušić Bojanac, Ana ; Bulic Jakus, Floriana ; Ježek, Davor ; Ulamec, Monika ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Andrology 2020 12th International/11th European/ 32nd German Congress of Andrology 5–9 December 2020 DIGITAL Abstracts / - Gablitz : Krause & Pachernegg GmbH, 2020, 32-33

Skup
International Congress of Andrology

Mjesto i datum
München, Njemačka, 05-09.12.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
teratoma, HDACi

Sažetak
Testicular Germ Cell Tumours (TGCT) are the most frequent malignancies in young males, driven by epimutations. Teratoma are TGCT composed of all three germ layer tissues. Mouse teratoma model, due to its heterogeneity and pluripotent core, is a very reliable in vitro model for investigating the effect of cancer drugs, including histone deacetylase inhibitors, on the epigenetic regulation of tumorigenicity. After isolation, mouse egg cylinders were treated for two hours with Trichostatin-A (TSA) and Valproate (VPA), respectively. Developing teratomas were cultured in vitro and measured for seven days. Proliferation (Ki-67) and apoptosis (Caspase-3) were analysed by immunohistochemistry. Gene expression of stemness/differentiation genes was analysed by qPCR. TSA and VPA induced strong growth retardation despite unchanged proliferative or apoptotic activity. Treatments exhibited opposite effects on gene expression compared to control. TSA upregulates while VPA downregulates stemness/differentiation genes. VPA downregulation of investigated genes could be responsible for reducing teratoma growth, initiating senescence. TSA upregulation of these genes points to a loss of expression regulation, with consequent growth retardation possibly being due to general teratoma dysregulation. This research confirms the effect of VPA and TSA strongly hampering cancer development. These treatments producing the same phenotypical effect of growth inhibition despite different molecular effects and no change in proliferative and mitotic activity illustrate the complex relationship between histone acetylation and regulation of biological processes. Grants This study was supported by the Scientific Center of Excellence for Reproductive and Regenerative Medicine, Republic of Croatia, and by the European Union through the European Regional Development Fund, under grant agreement No. KK.01.1.1.01.0008, project “Reproductive and Regenerative Medicine – Exploring New Platforms and Potentials”

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

www.kup.at

Citiraj ovu publikaciju:

Krasi, Jure; Škara, Lucija; Katušić Bojanac, Ana; Bulic Jakus, Floriana; Ježek, Davor; Ulamec, Monika; Sinčić, Nino
HDACi drugs hamper experimental mouse teratoma development // Andrology 2020 12th International/11th European/ 32nd German Congress of Andrology 5–9 December 2020 DIGITAL Abstracts
Gablitz: Krause & Pachernegg GmbH, 2020. str. 32-33 (poster, međunarodna recenzija, sažetak, znanstveni)
Krasi, J., Škara, L., Katušić Bojanac, A., Bulic Jakus, F., Ježek, D., Ulamec, M. & Sinčić, N. (2020) HDACi drugs hamper experimental mouse teratoma development. U: Andrology 2020 12th International/11th European/ 32nd German Congress of Andrology 5–9 December 2020 DIGITAL Abstracts.
@article{article, year = {2020}, pages = {32-33}, keywords = {teratoma, HDACi}, title = {HDACi drugs hamper experimental mouse teratoma development}, keyword = {teratoma, HDACi}, publisher = {Krause and Pachernegg GmbH}, publisherplace = {M\"{u}nchen, Njema\v{c}ka} }
@article{article, year = {2020}, pages = {32-33}, keywords = {teratoma, HDACi}, title = {HDACi drugs hamper experimental mouse teratoma development}, keyword = {teratoma, HDACi}, publisher = {Krause and Pachernegg GmbH}, publisherplace = {M\"{u}nchen, Njema\v{c}ka} }




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