engleski

The role of uroguanylin in ischemic stroke

AIM: Recent research has shown that the activation of guanylate cyclase (GC)-A leads to a decrease of brain lesion size following middle cerebral artery occlusion (MCAO). Our aim was to investigate the potential role of uroguanylin (UGN)-dependent signalling pathways in the development of ischemic stroke. METHODS: Lesion volumes were measured in WT, GC-C and UGN-KO mice by MR imaging 1 and 7 days after MCAO. Intracellular Ca2+ concentrations were measured in slices of ipsilateral and contralateral hemispheres. RESULTS: GC-C-KO animals exhibited significantly smaller lesion volumes 1 day after MCAO than WT mice, with no difference 7 days later. This difference was not present between UGN-KO and WT littermates, although there was significant difference in UGN- dependent activation of Ca2+ signalling pathway in penumbral zone of ischemic lesion. CONCLUSION: The reduction in stroke volume present in GC-C-KO but not UGN-KO mice indicates that the activation of GC-C may cause an increase in ischemic lesion volumes, while GC-C-independent (Ca2+-dependent) signalling pathway may have protective effect. ACKNOWLEDGEMENTS Research was funded by the Scientific Centre of Excellence for Basic, Clinical and Translational Neuroscience (project “Experimental and clinical research of hypoxic- ischemic damage in perinatal and adult brain” ; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund). The work of doctoral student Anja Barić has been fully supported by the “Young researchers' career development project – training of doctoral students” and project BRADISCHEMIA (UIP-2017-05- 8082) of the Croatian Science Foundation funded by the European Union from the European Social Fund.

guanylate cyclase-C ; middle cerebral artery occlusion ; MR imaging ; Ca2+ signaling

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