engleski

Increased inflammatory parameters, chemokines and chemokine receptors in obese children

Obesity represents a state of low‐grade chronic systemic inflammation, potentially leading to insulin resistance and endothelial damage, causing metabolic and vascular complications. We aimed to investigate metabolic and inflammatory parameters in obese children, by analyzing laboratory data and peripheral blood chemokine/chemokine receptor profile. Upon Ethical approval, mononuclear cells were isolated from peripheral blood of healthy controls (n=29, 14 M, age 15.46+/‐1.51 years) and obese children (n=34, 19 M, age 14.69+/‐ 1.60 years). B‐cell (CD19+), T‐cell (CD3+) and monocyte (CD14+) frequencies were determined in regard to chemokine receptor expression (CCR2, CCR4, CXCR3 and CXCR4) by flow cytometry. Chemokines (CCL2, CCL5, CXCL10, CXCL11) were determined by LEGENDplex™ and CXCL12 by ELISA. Data were correlated with clinical and laboratory parameters (HOMA‐ IR, ALT, lipid profile, diastolic blood pressure (DBP), CRP, fibrinogen). Obese children have higher inflammatory markers (CRP, fibrinogen) and parameters of metabolic complications (ALT, LDL‐cholesterol, triglycerides, DBP, HOMA IR). Their peripheral blood samples have decrease monocyte expression of CCR4 and CXCR3, and lower concentration of CXCL12. CXCR3+ monocytes showed negative correlation with CRP, fibrinogen, ALT, LDL‐cholesterol and HOMA‐IR, while CCR4+ monocytes positively correlated with HDL‐cholesterol, and negatively with HOMA‐IR and triglycerides. We also found correlation between CCR2+ B‐cells and HDL‐ cholesterol, and CCR4+ B‐calls and DBP. CCL2, CRP and fibrinogen positively correlated with metabolic parameters. Obesity is associated with systemic inflammation and metabolic complications even in young age. Precise identification of inflammatory molecules associated with these complications may indicate therapeutic targets for preventing obesity related morbidity and mortality.

B lymphocytes ; chemokines ; inflammatory disease ; inflammatory molecules ; myeloid cells

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