engleski

Yeast cell wall transcriptome

Yeast Saccharomyces cerevisiae cell wall is a strong, but flexible structure consisting mostly of β-1, 3- and β-1, 6-glucan, chitin and incorporated mannoproteins. This structure is continually remodeled as yeast cells undergo processes such as cell division, mating, meiosis and sporulation, which are therefore expected to be coupled with changes in expression of a certain subset of cell wall-related genes. Over the past decade, advances in high-throughput RNA-sequencing methods have enabled deep insight into complex yeast transcriptome. Many RNA transcripts, that are not translated into proteins and are therefore referred to as long non-coding RNAs (lncRNAs), have been found to play important functions in various cellular processes such as gene transcription, splicing and translation as well as nuclear organization, cell growth and developmental pathways. In our work we explore the cell wall transcriptome, which can be defined as the condition-specific cellular pool of mRNAs coding for proteins that are either incorporated in the cell wall or take part in biosynthesis and remodeling of the cell wall. We take advantage of high-throughput RNA-sequencing methods, as well as transcript-specific methods, to gain insight into how the cell wall transcriptome is modulated to support different life cycle phases and tolerate various environmental perturbations. The results of this work indicate that lncRNAs might regulate expression of certain cell wall-related genes.

yeast cell wall ; transcriptome ; non-coding RNAs

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