engleski

Untargeted metabolomic profiling of plasma from dogs with idiopathic dilated cardiomyopathy

Idiopathic dilated cardiomyopathy (iDCM) is the second most common cardiac disease in dogs, and the major cause of morbidity and mortality in various dog breeds. It is characterized by dilatation and impaired contraction of the left or both ventricles and carries a poor prognosis with progression to congestive heart failure or fatal arrhythmias.Plasma was collected from 8 dogs with iDCM and 12 healthy dogs. The plasma metabolome was analysed using untargeted metabolomics approach by applying UHPLC and Q Exactive Plus Orbitrap Mass Spectrometer operating in alternating positive and negative modes with mass resolution 70.000 at m/z range 70-1050. Analyses were performed using Polyomics integrated Metabolomics Pipeline (PiMP) program specifically designed for metabolomics.Metabolomics analysis resulted in the annotation of 2636 peaks, 237 of which showed statistically significant differences (p<0.05) between dogs with iDCM and healthy control. Among them, 48 metabolites were identified using standards. When the two groups were compared, the most significant metabolites are O-Acetylcarnitine, L-Carnitine and cis- Aconitic acid.The study shows that the differences in plasma metabolome between dogs with iDCM and healthy dogs can be detected with an untargeted metabolomics approach using UHPLC-MS/MS method. O- Acetylcarnitine, L-Carnitine, and cis-Aconitic acid may have potential in the discovery of biomarkers relevant for iDCM.

Idiopathic dilated cardiomyopathy ; plasma samples ; metabolomics

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