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Pregled bibliografske jedinice broj: 1161752

Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets


Bura, Ana; Jurak Begonja, Antonija
Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets // Life, 11 (2021), 12; 1331, 20 doi:10.3390/life11121331 (međunarodna recenzija, članak, znanstveni)


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Naslov
Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets

Autori
Bura, Ana ; Jurak Begonja, Antonija

Izvornik
Life (2075-1729) 11 (2021), 12; 1331, 20

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
platelets ; phosphatidylinositol-4, 5-bisphosphate ; phosphatidylinositol-4-monophosphate ; immunofluorescence ; plasma membrane

Sažetak
Phosphoinositides (PIs) are phosphorylated membrane lipids that have a plethora of roles in the cell, including vesicle trafficking, signaling, and actin reorganization. The most abundant PIs in the cell are phosphatidylinositol- 4, 5-bisphosphate [PI(4, 5)P2] and phosphatidylinositol-4-monophosphate (PI4P). The localization and roles of both PI(4, 5)P2 and PI4P are well established, is the broadly accepted methodological approach for their immunocytochemical visualization in different cell compartments in several cell lines. However, not much is known about these PIs in platelets (PLTs), the smallest blood cells that detect vessel wall injury, activate, and stop the bleeding. Therefore, we sought to investigate the localization of PI(4, 5)P2 and PI4P in resting and activated PLTs by antibody staining. Here, we show that the intracellular pools of PI(4, 5)P2 and PI4P can be detected by the established staining protocol, and these pools can be modulated by inhibitors of OCRL phosphatase and PI4KIIIα kinase. However, although resting PLTs readily stain for the plasma membrane (PM) pools of PI(4, 5)P2 and PI4P, just a few activated cells were stained with the established protocol. We show that optimized protocol allows for the visualization of PI(4, 5)P2 and PI4P at PM in activated PLTs, which could also be modulated by OCRL and PI4KIIIα inhibitors. We conclude that PI(4, 5)P2 and PI4P are more sensitive to lipid extraction by permeabilizing agents in activated than in resting human PLTs, which suggests their different roles during PLT activation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
NadSve-Sveučilište u Rijeci-uniri-biomed-18-188-1343 - Identifikacija novih interakcijskih partnera Vps34 u megakariopoezi (Jurak Begonja, Antonija, NadSve - UNIRI projekti 2018) ( POIROT)
HRZZ-UIP-2014-09-2400 - Uloga fosfoinozitida u nastanku trombocita (MkPI) (Jurak Begonja, Antonija, HRZZ - 2014-09) ( POIROT)
Ostalo-ASH-2019 - Nucleolar control of megakaryopoiesis (NucMeg) (Jurak Begonja, Antonija, Ostalo - American Society of Hematology (ASH) Global Research Award) ( POIROT)
Ostalo-CRP/HRV15-04_EC - Deciphering the role of phosphoinositol lipids in thrombopoiesis (Jurak Begonja, Antonija, Ostalo - CRP-ICGEB Early Career Return Grant) ( POIROT)

Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju

Profili:

Avatar Url Ana Bura (autor)

Avatar Url Antonija Jurak Begonja (autor)

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Bura, Ana; Jurak Begonja, Antonija
Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets // Life, 11 (2021), 12; 1331, 20 doi:10.3390/life11121331 (međunarodna recenzija, članak, znanstveni)
Bura, A. & Jurak Begonja, A. (2021) Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets. Life, 11 (12), 1331, 20 doi:10.3390/life11121331.
@article{article, author = {Bura, Ana and Jurak Begonja, Antonija}, year = {2021}, pages = {20}, DOI = {10.3390/life11121331}, chapter = {1331}, keywords = {platelets, phosphatidylinositol-4, 5-bisphosphate, phosphatidylinositol-4-monophosphate, immunofluorescence, plasma membrane}, journal = {Life}, doi = {10.3390/life11121331}, volume = {11}, number = {12}, issn = {2075-1729}, title = {Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets}, keyword = {platelets, phosphatidylinositol-4, 5-bisphosphate, phosphatidylinositol-4-monophosphate, immunofluorescence, plasma membrane}, chapternumber = {1331} }
@article{article, author = {Bura, Ana and Jurak Begonja, Antonija}, year = {2021}, pages = {20}, DOI = {10.3390/life11121331}, chapter = {1331}, keywords = {platelets, phosphatidylinositol-4, 5-bisphosphate, phosphatidylinositol-4-monophosphate, immunofluorescence, plasma membrane}, journal = {Life}, doi = {10.3390/life11121331}, volume = {11}, number = {12}, issn = {2075-1729}, title = {Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets}, keyword = {platelets, phosphatidylinositol-4, 5-bisphosphate, phosphatidylinositol-4-monophosphate, immunofluorescence, plasma membrane}, chapternumber = {1331} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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