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Pregled bibliografske jedinice broj: 1161748

Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy


Mikulandra, Ivana; Kušec, Iva; Jednačak, Tomislav; Novak, Predrag
Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy // Euromar Portorož 2021 : Book of abstracts
Portorož, Slovenija, 2021. str. 216-216 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 1161748 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy

Autori
Mikulandra, Ivana ; Kušec, Iva ; Jednačak, Tomislav ; Novak, Predrag

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Euromar Portorož 2021 : Book of abstracts / - , 2021, 216-216

Skup
Euromar Portorož 2021

Mjesto i datum
Portorož, Slovenija, 05-08.07.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
azithromycin, interactions, macrolides, spectroscopy NMR

Sažetak
Azithromycin is a semisynthetic macrolide antibiotic which possess excellent pharmacokinetic properties and demonstrates satisfactory biological effect. Macrolides are bacteriostatic antibiotics. They exert their biological activity by binding to the 50S subunit of the ribosome. Macrolides are effective against Gram-positive and some Gram-negative bacterial strains [1, 2]. Due to the frequent and improper use of drugs bacterial resistance has emerged, so there is a need to discover more effective antibiotics. Azithromycin aminopropyl derivatives are precursors in synthesis of macrozones. Macrozones are novel bioactive macrolide derivatives and conjugates of azithromycin and thiosemicarbazones [3]. In order to fully understand the mechanism of macrozone bioactivity it is important to determine their bound conformation and interactions with biological targets such as ribosome [4]. In our study, we used NMR spectroscopy to analyse binding of azithromycin derivatives with ribosome isolated from Escherichia coli. Conformations in free and bound state were studied by NOESY (nuclear Overhauser effect spectroscopy) and trNOESY (transferred nuclear Overhauser effect spectroscopy) experiments. STD NMR (Saturation transfer difference Spectroscopy) was used to determine the binding epitopes of azithromycin derivatives. By these techniques relationship between structure and activity will be explored. These results should contribute to the discovery and design of new, more effective macrolide antibiotics.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
HRZZ-IP-2018-01-8098 - Makrozoni, novi konjugati makrolidnih antibiotika: Dizajn, priprava i interakcije (Makrozoni/Macrozones) (Novak, Predrag, HRZZ - 2018-01) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Tomislav Jednačak (autor)

Avatar Url Ivana Mikulandra (autor)

Avatar Url Predrag Novak (autor)


Citiraj ovu publikaciju:

Mikulandra, Ivana; Kušec, Iva; Jednačak, Tomislav; Novak, Predrag
Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy // Euromar Portorož 2021 : Book of abstracts
Portorož, Slovenija, 2021. str. 216-216 (poster, nije recenziran, sažetak, znanstveni)
Mikulandra, I., Kušec, I., Jednačak, T. & Novak, P. (2021) Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy. U: Euromar Portorož 2021 : Book of abstracts.
@article{article, author = {Mikulandra, Ivana and Ku\v{s}ec, Iva and Jedna\v{c}ak, Tomislav and Novak, Predrag}, year = {2021}, pages = {216-216}, keywords = {azithromycin, interactions, macrolides, spectroscopy NMR}, title = {Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy}, keyword = {azithromycin, interactions, macrolides, spectroscopy NMR}, publisherplace = {Portoro\v{z}, Slovenija} }
@article{article, author = {Mikulandra, Ivana and Ku\v{s}ec, Iva and Jedna\v{c}ak, Tomislav and Novak, Predrag}, year = {2021}, pages = {216-216}, keywords = {azithromycin, interactions, macrolides, spectroscopy NMR}, title = {Interactions of azithromycin aminopropyl derivatives with E. coli ribosome studied by NMR spectroscopy}, keyword = {azithromycin, interactions, macrolides, spectroscopy NMR}, publisherplace = {Portoro\v{z}, Slovenija} }




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