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Cytotoxic Activity of Ferrocene-substituted Purines Against Several Cancer Cell Lines (CROSBI ID 711250)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Toma, Mateja ; Marjanović Čermak, Ana Marija ; Pavičić, Ivan ; Vinković Vrček, Ivana ; Vrček, Valerije Cytotoxic Activity of Ferrocene-substituted Purines Against Several Cancer Cell Lines // 5th Mini Symposium of Section of Medicinal and Pharmaceutical Chemistry, Book of Abstracts / Gabelica Marković, Vesna (ur.). Zagreb: Hrvatsko kemijsko društvo, Sekcija za medicinsku i farmacutsku kemiju, 2021. str. 15-15

Podaci o odgovornosti

Toma, Mateja ; Marjanović Čermak, Ana Marija ; Pavičić, Ivan ; Vinković Vrček, Ivana ; Vrček, Valerije

engleski

Cytotoxic Activity of Ferrocene-substituted Purines Against Several Cancer Cell Lines

A series of ferrocene-substituted purines with carbonyl group as linker between the organometallic and heterocyclic part were synthesized by regioselective acylation [1] and their cytotoxic activity against several cancer cell lines was investigated. Since ferrocenyl compounds are shown to exhibit diverse biological activity through redox mechanism by generating reactive oxygen species (ROS) [2], electrochemical and ROS generation studies were carried out. Cyclic voltammetry showed that all measured compounds follow a reversible one-electron oxidation in the range of 300-450 mV with the N7 isomers being better oxidants than the N9. While nucleobases coupled with ferrocene generate ROS in acellular media, ferrocene and nucleobases itself were not active. Cytotoxic effect against L929 representing normal cells, and Panc-1, A549 and MCF-7 cancer cell lines was determined based on IC50 values using MTT viability assay. N7 and N9 isomers containing fluorine atom at position 2 and amino group at position 6 of the purine ring, were the most active against all examined cell lines with IC50 value between 1 and 5 µM. Other compounds showed higher IC50 values (50-200 µM). The oxidative status of cells treated with compounds showing low IC50 values was assessed by cellular ROS generation with DCFH reagent and glutathione production with monochlorobimane. Collected data showed no correlation between cytotoxic effect and acellular ROS generation and/or the redox potential of ferrocene-substituted purines.

ferrocene-substituted purines, redox potential, ROS, cytotoxic effect, cancer cell lines

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Podaci o prilogu

15-15.

2021.

objavljeno

Podaci o matičnoj publikaciji

5th Mini Symposium of Section of Medicinal and Pharmaceutical Chemistry, Book of Abstracts

Gabelica Marković, Vesna

Zagreb: Hrvatsko kemijsko društvo, Sekcija za medicinsku i farmacutsku kemiju

Podaci o skupu

5th Mini Symposium of Section of Medicinal and Pharmaceutical Chemistry

predavanje

30.11.2021-30.11.2021

Zagreb, Hrvatska

Povezanost rada

Farmacija, Kemija

Poveznice