engleski

RAPID AND SIMPLE MICROFLUIDIC METHODS FOR DETECTION OF BIOACTIVE COMPOUNDS IN COMPLEX SAMPLES

In modern nutrition research amino acids and peptides have a broad potential therapeutic applications, and are used as supplements or as functional food ingredients. β-alanine, Lhistidine and carnosine are common used in the form of dietary supplements. Carnosine is dipeptide produced by condensation of β-alanine and L-histidine.[1] Standard methods for analysis of amino acid and peptides have a number of disadvantages. They are expensive, complex and time consuming. Microchip electrophoresis (ME) evolved from capillary electrophoresis with purpose of reducing the time and cost of analyzes, the amount of reagents, samples and waste.[2] ME with capacitively-coupled contactless conductivity detection allows fast and environmentally friendly analysis of ions and ionisable molecules in complex samples. During the study various parameters was investigated to provide high resolution and to optimize the separation. A key point was to optimize the separation buffer in order to avoid overlapping of the amino acids with the other constituents in the analyzed sample. The detection limits were below 1 mg/mL. The proposed microfluidic methods are environmentally friendly and offer great promises for routine multi-analyte pharmaceuticals and food analyses.

β-alanine, Amino acids, Functional food, Microfluidics, Supplements,

nije evidentirano